1999 年 58 巻 2 号 p. 71-77
To clarify sexual maturation in the land snail, Euhadra peliomphala, seasonal changes in some items of the albumen gland were examined. No clear differences were detected in the shell size and whole body weight. The weight of the albumen gland and the ratio of the albumen gland weight to the whole body weight showed relatively high values from spring to summer, and low values from autumn to winter in the semi-laboratory condition. Further, soluble proteins extracted from the albumen gland in each season were quantified, and were subjected to polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS-PAGE). Levels of soluble proteins in the snails in May were 3-fold larger than those in the snails in March. SDS-PAGE showed clear differences of the protein composition in each season. The 68k protein seemed to be synthesized from spring to summer. In conclusion, the present study clearly showed the seasonal changes appeared in the weight of the albumen gland, the ratio of the albumen gland weight to the whole body weight and the protein contents of the albumen gland in E. peliomphala.