Abstract
This paper deals with the role of some phosphates such as glucose-1-phosphate, glucose-6-phosphate and β-glycerophosphate in the formation of riboflavin by Escherichia coli communior. The effect of each phosphate was examined in adding to the basal medium in the amount of 1/3000 mole or 200mg per ml in place of g1ucose. The results obtained were as follows : (1) Contrary to expectation, each compound above mentioned was less effective than glucose in the biosynthesis of riboflavin. The highest formation of the vitamin was observed in the addition of glucose-1-phosphate of the three compounds and the least in β-glycerophosphate. (2) It was suggested that some of the energy produced in glycolysis might be utilized for the biosynthesis of riboflavin.
