Abstract
Various methods for the release of folic acid and citrovorum factor from their bound forms in human blood were reexamined, and the folic acid and citrovorum factor levels in blood were estimated by the differential microbiological analysis with Streptococcus faecalis and Leuconostoc citrovorum as test organisms. From the data, it was concluded that the enzymatic treatment of blood with hog kidney and chicken pancreas preparations activated by cysteine was the most effective method for the release of folic acid and citrovorum factor from their conjugated forms in blood. With the aid of this treatment, the blood levels of folic acid and citrovorum factor were determined on normal adults. The mean leveles were 8.6±1.6 mγ/ml for folic acid, 2.5±0.9 mγ/ml for citrovorum factor.
