Abstract
Author has already reported that 2-ketogulonic acid methylester turned into a vitamin C-like substance in the presence of the extract of animal tissues, and this substance considered to be ascorbic acid. In this paper the ultraviolet absorption spectrum of this substance was discussed. 1) By means of paper chromatography and ultraviolet spectroscopy, with solutions of pH 2 and 5,this substance was identified with ascorbic acid. 2) Ultraviolet absorption spectrum of this substance was disappeared in the presence of Cu ion in alkaline medium.
