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Toichiro SAWADA
Article type: Article
1956 Volume 11 Pages
1-5
Published: 1956
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1956 Volume 11 Pages
5-
Published: 1956
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1956 Volume 11 Pages
5-
Published: 1956
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1956 Volume 11 Pages
5-
Published: 1956
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1956 Volume 11 Pages
5-
Published: 1956
Released on J-STAGE: December 22, 2017
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Minoru OTA
Article type: Article
1956 Volume 11 Pages
6-12
Published: 1956
Released on J-STAGE: December 22, 2017
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F. Aoki clarified that thiaminase II carrying yeast-like fungi isolated from the oral cavity belonged to genus Trickosporon. Further investigations were made on the physiological and morphological characters of this strain, and it was concluded that it is a related strain of Trichosporon cutaneum.
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Minoru OTA
Article type: Article
1956 Volume 11 Pages
13-19
Published: 1956
Released on J-STAGE: December 22, 2017
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Examining the vitamin requirement of a thiaminase II carrying Trichosporon YA strain, it was found that this strain required thiamine as an essential growth factor.
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Hideo NAKAYAMA
Article type: Article
1956 Volume 11 Pages
20-25
Published: 1956
Released on J-STAGE: December 22, 2017
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In the case of a wild-strain of E. coli ATCC 9637,the concentration of thiamine-thiazole required to reverse inhibition showed a competitive relationship with the concentration of "amino-thiazole" present in a minimal medium. The growth of a mutant 70-23,which requires pre-formed thiamine, was not appreciably, inhibited by amino-thiazole under the condition which permitted its growth to a limited extent. The mutant 26-43,whose genetic deficiency is characterized as an inability of carrying out thiazole synthesis while its synthetic ability of thiamine in the conjugation of pyrimidine and thiazole is retained as well as in the wild-strain, was slightly more susceptible to amino-thiazole than the wild-strain. Among thiamine-requiring strains, 70-17 which was previously indicated to have been blocked partially in the conjugation of the two moieties of thiamine, was nhibited by the lowest level of amino-thiazole. For securing particular classes of auxotrophs from a wild-strain, it is most important that nutrients excreted from prototrophs are not utilized by auxotrophs during penicillin screening. Isolation of thiamine-requiring mutants, partially blocked in the reaction for thiamine synthesis was easily permitted by a selection in a penicillin medium containing a certain concentration of amino-thiazole. This success may be due to the avoidance of syntrophic sterilization of desired type of mutants by the presence of the anti-metabolite, to which the mutant is more susceptible than the parent organism.
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[in Japanese]
Article type: Article
1956 Volume 11 Pages
25-
Published: 1956
Released on J-STAGE: December 22, 2017
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Keisuke YASHIMA
Article type: Article
1956 Volume 11 Pages
26-29
Published: 1956
Released on J-STAGE: December 22, 2017
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Author has already reported that 2-ketogulonic acid methylester turned into a vitamin C-like substance in the presence of the extract of animal tissues, and this substance considered to be ascorbic acid. In this paper the ultraviolet absorption spectrum of this substance was discussed. 1) By means of paper chromatography and ultraviolet spectroscopy, with solutions of pH 2 and 5,this substance was identified with ascorbic acid. 2) Ultraviolet absorption spectrum of this substance was disappeared in the presence of Cu ion in alkaline medium.
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[in Japanese]
Article type: Article
1956 Volume 11 Pages
29-
Published: 1956
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1956 Volume 11 Pages
29-
Published: 1956
Released on J-STAGE: December 22, 2017
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Kengo OKUMURA, Yousuke ASANO, Yosito SAKURAI
Article type: Article
1956 Volume 11 Pages
30-35
Published: 1956
Released on J-STAGE: December 22, 2017
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(1) Both disulfide type compounds and O, S-diacyl compounds of thiamine give a considerable coloration in Prebluda-McCollum reaction. (2) It was realized that Prebluda-McCollum reaction of the disulfide type compounds of thiamine is caused by the conversion of them to thiamine with alkali in the reagent.
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Ryohei TAKATA, Chikahiro SAKAZAWA, Kinji TANAKA
Article type: Article
1956 Volume 11 Pages
35-39
Published: 1956
Released on J-STAGE: December 22, 2017
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Studies on the manufacture of crystalline vitamin B_<12> from activated sludge are now in progress in our laboratory. On the other hand, a search is made for vitamin B_<12> resources in Japan other than the activated sludge. The supernatant liquor running from the night soil digestion plant was found to be a superior raw material for the preparation of vitamin B_<12> to the activated sludge. The vitamin content of the solid portion obtained by centrifugation of the former (composed chiefly of bacterial cells) was 30-40 μg/g on dry basis, while that of activated sludge was 4-8 μg/g.
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[in Japanese]
Article type: Article
1956 Volume 11 Pages
39-
Published: 1956
Released on J-STAGE: December 22, 2017
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Ryohei TAKATA, Chikahiro SAKAZAWA, Kinji TANAKA
Article type: Article
1956 Volume 11 Pages
40-43
Published: 1956
Released on J-STAGE: December 22, 2017
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The night soil digestion plant in Tokyo is the largest one in Japan, where a thousand cubic meters of night soil is treated daily. The vitamin B_<12> content of centrifuged portion of the supernatant liquor from the plant was found to be much lower than that previously observed at the Kotoura plant, namely 9-17 μg/g on dry basis. Examinations were also made at the plants in Suwa and Kobe. The centrifuged portion of the supernatant liquor contained about 30 μg/g in the former and only about 10 μg/g in the latter.
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Tadashi KAMIKUBO
Article type: Article
1956 Volume 11 Pages
43-45
Published: 1956
Released on J-STAGE: December 22, 2017
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The microbiological tube assay of vitamin B_<12> with a mutant of E. coli produced by Hayashi as a test organism was discussed. Determinations carried out on activated sludge, digested sludge and anaerobic fermentation products from distillers' plants indicate that E. coli mutant method gives results which are comparable to those obtained with L. leichmannii method. The former is also better in much lower expenses for basal medium as well as in good response to vitamin B_<12>.
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[in Japanese]
Article type: Article
1956 Volume 11 Pages
45-
Published: 1956
Released on J-STAGE: December 22, 2017
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Narimasa OOI
Article type: Article
1956 Volume 11 Pages
46-48
Published: 1956
Released on J-STAGE: December 22, 2017
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For the determination of pteroylglutamic acid excreted in the 24 hour urine after an oral dose of 10 mg of pteroylglutamic acid, a simple and rapid method was reported, which was a modification of Allfrey et al.'s. In this mothod, pteroylglutamic acid in the urine was precipitated with lead acetate and eluated in ammonium sulfate solution, then oxidized by potassium permanganate, and finally meassured fluorometrically. The pteroylglutamic acid added to urine was recovered satisfactorily by this method.
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Narimasa OOI
Article type: Article
1956 Volume 11 Pages
48-51
Published: 1956
Released on J-STAGE: December 22, 2017
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Macrocytic anemia was found in 16 out of the 19 children with nutritional dystrophy. Pteroylglutamic acid excretion in the 24 hour urine after an oral dose of 10 mg of pteroylglutamic acid was decidedly lower in the majority of the children with nutritional dystrophy than in the normal subjects. The existence of folic acid deficiency in the children with nutritional dystrophy was suggested from the low excretion of pteroylglutamic acid after oral dose as well as high incidence in macrocytic anemia among these children.
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[in Japanese]
Article type: Article
1956 Volume 11 Pages
51-
Published: 1956
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1956 Volume 11 Pages
51-
Published: 1956
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1956 Volume 11 Pages
51-
Published: 1956
Released on J-STAGE: December 22, 2017
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Kazuo KINUGASA
Article type: Article
1956 Volume 11 Pages
52-57
Published: 1956
Released on J-STAGE: December 22, 2017
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Electromyographic studies were conducted in acute poliomyelitis patients and in experimentally infected poliomyelitis monkeys. Follwing injection of glutamylcholine, discharge of motor unit voltages approaches normal. It is suggested that muscle function is restored by the injection of glutamylcholine.
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Shinichi BAMBA
Article type: Article
1956 Volume 11 Pages
58-82
Published: 1956
Released on J-STAGE: December 22, 2017
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Glutamylcholine was administered in 158 cases of hypertension and the effect on the blood pressure, capillary resistance, capillary net-work of the skin, blood and urine picture, serum viscosity, fundus of the eye and electrocardiogram was examined. A favorable effect was noted. In an attempt to clarify the mechanism of action from the viewpoint of metabolism, studies were conducted on changes in the total protein, albumin, globulin, fat, chloride, sodium, potassium and choline esterase of the serum.
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[in Japanese]
Article type: Article
1956 Volume 11 Pages
82-
Published: 1956
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1956 Volume 11 Pages
82-
Published: 1956
Released on J-STAGE: December 22, 2017
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Naotane MIYAZAKI
Article type: Article
1956 Volume 11 Pages
83-94
Published: 1956
Released on J-STAGE: December 22, 2017
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The administration of glutamylcholine together with cholesterol in the rabbit suppresses the occurrence of hypertension and arteriosclerosis. The administration of glutamylcholine in the hypertension, experimentally induced in the rabbit by administration of cholesterol, resulted in a lowering of the blood pressure. No therapeutic effect could, however, be seen on the arteriosclerosis which had already set in, but a decrease in the fat deposition in various organs and small vessels was observed. The administration of cholestrol alone resulted in a picture of marked dysfunction of the adrenal cortex and decrease in function of the gonads. On the other hand, the changes in the adrenal cortex were milder and the gonads showed a grossly normal picture when glutamylcholine was given concomitantly. It is suggested from these facts that the action of glutamylcholine on blood pressure may be related to the metabolism of fat and to the endocrine system.
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[in Japanese]
Article type: Article
1956 Volume 11 Pages
94-
Published: 1956
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1956 Volume 11 Pages
94-
Published: 1956
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1956 Volume 11 Pages
94-
Published: 1956
Released on J-STAGE: December 22, 2017
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Fumito SHIMOMURA
Article type: Article
1956 Volume 11 Pages
95-102
Published: 1956
Released on J-STAGE: December 22, 2017
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Washed cells of Micrococcus pyogenes can decompose thiamine to its moieties. The rate of the decomposition was compared with that of thiamine synthesis and the mechanisms of the two acts were discussed.
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[in Japanese]
Article type: Article
1956 Volume 11 Pages
102-
Published: 1956
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1956 Volume 11 Pages
102-
Published: 1956
Released on J-STAGE: December 22, 2017
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Koichi SAITO
Article type: Article
1956 Volume 11 Pages
103-110
Published: 1956
Released on J-STAGE: December 22, 2017
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Froliferation or atrophy of lingual papilla, red or violet-red color, fissure formation and edematous appearance of the tongue, and stomatitis angularis were observed, and each of the signs was proved to be in a close relationship with riboflavin deficiency. 1) The average value of blood total riboflavin of 103 patients who showed most of the signs above mentioned, was 5.4±0.5 μg%, while it was 7.4±1.2 μg% in 46 healthy subjects. This difference is stochastically significant. 2) The average value of blood total riboflavin of each group of patients showing some single sign, irrespective of its kind, was also found to be lower than that of healthy group. 3) Reverse correlation with stochastical significance was found to exist between the number of the lingual signs and the value of blood total riboflavin. 4) Urinary excretion of riboflavin appeared to bear no direct relationship with these lingual signs.
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Koichi SAITO
Article type: Article
1956 Volume 11 Pages
110-116
Published: 1956
Released on J-STAGE: December 22, 2017
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Attempt was made to acknowledge the importance of furring for diagnosis of riboflavin-deficiency by determining total and esterified riboflavin in the blood, and observing lingual ariboflavinotic signs and angular stomatitis. 1) The patients showing these signs were divided into two groups, one with furring (group A) and the other without it (group B). The total blood riboflavin of the latter was lower than that of healthy persons, but that of the former was not aways so. 2) With regard to the amount of blood esterified riboflavin, it was found, as was expected, that it was lower in group B than in healthy subjects, but it should be emphasized that this was also the case in group A. The real deficient state in group A may therefore be regarded as to have been masked by the existence of free riboflavin. 3) The above results reveal that grout B is superior to group A in the degree of riboflavin-deficiency and that furring may be used as an indicator for judging the grade of deficiency.
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Koichi SAITO
Article type: Article
1956 Volume 11 Pages
117-124
Published: 1956
Released on J-STAGE: December 22, 2017
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Variations in the electrophoretic pattern of plasma protein were studied with patients showing lingual signs or angular stomatitis related to riboflavin-deficiency, and following results were obtained. 1) Low albumin, high γ-globulin and low albumin-globulin ratio were found to be closely correlated with the above mentioned signs, both developing with increase in riboflavin-deficiency. These variation were more marked in the patients showing furring on their tongues (group A) than in those without it (group B). 2) Low albumin and albumin-globulin ratio and high γ-globulin were more marked in the patients showing atrophy of papillae, edema, violet-red coloring, fissure formation on the tongue and angular stomatitis and less marked among those showing red-coloring or proliferation of papillae. These variation were more eminent in group B than in group A. 3) In group B, the ester ratio of riboflavin or riboflavin-ester concentration of blood was found to correlate with albumin concentration and reversely with γ-globulin. In group A the correlation between the ester ratio and albumin concentration only was found. 4) Riboflavin deficiency was evidenced to be related closely with liver damages and hypoproteinemia. So it should be kept in mind that a vicious circle will be effected between the riboflavin deficiency and the liver damage or hypoproteinemia.
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Koyata HAMADA, Masami SASAKI, Kin-ichi YOSHIMURA
Article type: Article
1956 Volume 11 Pages
124-132
Published: 1956
Released on J-STAGE: December 22, 2017
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Two new kinds of riboflavin decomposing bacteria, strains 14-I and A-I, were isolated from human feces. Both organisms are Gram-positive, non-sporeforming and of motile rods, but differ from each other immunologically and bacteriologically in detail and also from all the other known riboflavin decomposing bacteria. Strain 14-I decomposed riboflavin intensely, whereas A-I did feebly. But the decomposition by A-I was much augmented when cultured mixed with a certain bacterium, strain A-IV, A-IV, though isolated from the same feces as A-I was done, is a Gram-positive, non-sporeforming and of motile rod, but showed no ability to decompose riboflavin in its sole culture. In either 14-I or A-I and A-IV, the decomposition was proved to produce lumichrome, and revealed to be promoted by shake culture, but it did not occur at all when glucose was added to culture medium.
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[in Japanese]
Article type: Article
1956 Volume 11 Pages
132-
Published: 1956
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1956 Volume 11 Pages
132-
Published: 1956
Released on J-STAGE: December 22, 2017
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Syoichi SHIMIZU
Article type: Article
1956 Volume 11 Pages
133-141
Published: 1956
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1956 Volume 11 Pages
141-
Published: 1956
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1956 Volume 11 Pages
142-
Published: 1956
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1956 Volume 11 Pages
142-
Published: 1956
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1956 Volume 11 Pages
142-143
Published: 1956
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1956 Volume 11 Pages
143-
Published: 1956
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese]
Article type: Article
1956 Volume 11 Pages
143-
Published: 1956
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1956 Volume 11 Pages
143-144
Published: 1956
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese]
Article type: Article
1956 Volume 11 Pages
144-
Published: 1956
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1956 Volume 11 Pages
144-
Published: 1956
Released on J-STAGE: December 22, 2017
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