CHEMICAL STUDIES ON FLAVINADENINEDINUCLEOTIDE RELA : (II) TION BETWEEN THE CONCENTRATION OF FLAVINADE-NINEDINUCLEOTIDE AND ITS FLUORESCENCE INTENSITY

Abstract

The author tried to analyze mathematically the intensity of the fluorescence of flavinadeninedinucleotide using Kavanagh's equation, and obtained the following conclusions : (1) Only in dilute solutions of flavinadeninedinucleotide less than 1 μg/ml, the intensity of fluorescence was proportional to the concentration, therefore the fluorometric determination of it should be done in this range.(2) Putting the intensity of fluorescence of 10 μg/ml solution at 100,that of 50 μg/ml was 149,and no further increase was found by increasing the concentration over this level.