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Ryohei TAKATA
Article type: Article
1957 Volume 12 Pages
1-10
Published: 1957
Released on J-STAGE: December 22, 2017
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Norio SHIMAZONO, Shigeo HORIE, Kuniko OKOSHI, Tsuneki SHINOHARA, Eiji ...
Article type: Article
1957 Volume 12 Pages
11-19
Published: 1957
Released on J-STAGE: December 22, 2017
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The bakers' yeast fermenting with the maximum rate under suitable conditions shows still more carbon dioxide production provided that a minute amount of thiamine is added. This phenomenon was discovered by Atkin, Schultz, and Frey, and was applied for microdetermination of this vitamin. By use of this determination method, the authors examined the accelerating effect of thiamine and twenty-three kinds of its derivatives on anaerobic carbon dioxide production, and found that this method could be used as an examination method for the biological activity of thiamine analogues. However, care must be taken of the fact that the effect on yeast do not always agree with the effect on animals. Among the thiamine analogues tested, 1) Analogues of S, S-alkyl type were found to be nearly as active as thiamine, and some of them were slightly more active than thiamine. 2) When the analogues of O, S-diacyl type were examined, the effect of O, S-diacetyl thiamine was found to be almost the same as that of thiamine. Among the following compounds, the full developement of the effect was delayed in case of the later-cited compound compared with the former-cited. O, S-diacetyl>;dipropionyl>;propionyl-benzoyl>;dibenzoyl However, after the full developement of the accelerating effect, all of these analogues were as effective as thiamine. 3) O-acyl analogues were slightly less effective than thiamine. 4) S-alkyl analogues were not effective in general. 5) Thiothiamine and its derivatives did not show any activity. 6) Three kinds of antivitamins were tested, but among these only oxythiamine exhibited a slight anti-thiamine activity. As to the mechanism of the acceleration, it was assumed that the nitrogen metabolism had an essential interrelationship with the effect. It was observed that the nitrogen source was indispensable for the development of the effect, and the increase in nitrogen content and sporation rate of yeast cells accompanied with the increase of the effect. Metal ions and the cell-membrane permeability also played some role for the development of the accelerating effect.
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[in Japanese]
Article type: Article
1957 Volume 12 Pages
19-
Published: 1957
Released on J-STAGE: December 22, 2017
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Toshio NAKABAYASHI
Article type: Article
1957 Volume 12 Pages
20-24
Published: 1957
Released on J-STAGE: December 22, 2017
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In order to study the thermostable antithiamine factor (SF) of horsetail, the thiamine decomposing activities of paper chromatograms of horsetail extract were estimated and it was found that two pigments and some fluorescent substances had a strong activity. From ethyl acetate fraction of alcoholic extract of horsetail, yellow needle crystals, i. e., articulatin (C_<20>H_<18>O_<12>・2H_2O, m.p. 191°), was isolated. When articulatin was hydrolysed, yellow needles, i e., articulatidin (C_<l4>H_6O_7・l^I/2H_2O, m.p. 291-293°), and glucose were produced. It is, therefore, supposed that articulatin was pentahydroxyanthraquinone-glucoside. Because articulatin and articulatidin had a strong thiamine decomposing activity, a part of SF of horsetail was considered to be formed of these pigments.
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Kazunori TSUKIHARA, Masakazu IZUMIYA
Article type: Article
1957 Volume 12 Pages
24-31
Published: 1957
Released on J-STAGE: December 22, 2017
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The following results were obtained by a submerged culture of Eremothecium ashbyii using a fermentor of 100 liters capacity : 1) Riboflavin of 50-100mg% were obtained on a medium containg glucose and peptone as main substrates ; the maximum yield was 109mg%. 2) On a medium consisting of cane molasses and fish meals chiefly, riboflavin yields were 100-150 mg% but the maximum yield was found to be 217mg%.
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Kazunori TSUKIHARA, Masakazu IZUMIYA, Tadao KUSUMOTO
Article type: Article
1957 Volume 12 Pages
31-35
Published: 1957
Released on J-STAGE: December 22, 2017
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1. In industrial tests using a fermenter of two tons in capacity, the authors obtained nearly the same results as the previous findings with a 100 liters plant ; namely with a medium consisting of cane molasses and peptone, or cane molasses and fish meal, riboflavin yields in the method of feeding were of 127-216mg%. 2. From cost accounting with necessary industrial plans, it was concluded that the economical scale of production should be over 500 kg of riboflavin per month, and that it is premature to materialize such methods as the amount of consumption of riboflavin is not so high in Japan.
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[in Japanese]
Article type: Article
1957 Volume 12 Pages
35-
Published: 1957
Released on J-STAGE: December 22, 2017
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Kazunori TSUKIHARA, Kotoyuki YOSHITAKE
Article type: Article
1957 Volume 12 Pages
36-39
Published: 1957
Released on J-STAGE: December 22, 2017
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Investigations were made on all the processes of preparing riboflavin from the cultured broth containing cane molasses and fish meal as main substrates, and the following results were obtained : 1) By separation of solids from the broth, a crude riboflavin solution containing 91% of riboflavin was obtained. 2) Less than 5 % of riboflavin was lost by the deproteinization with 1 % of alum added to a solution containing 100 mg % or less of riboflavin. 3) Reduced riboflavin was obtained in the yield of 93 % by the addition of sodium hydrosulfite, three times as much as riboflavin contained in the solution. 4) After oxidation of reduced riboflavin precipitate, riboflavin was recrystallized and obtained in the yield of 80 %. This yield corresponded approximately to 70 % of riboflavin contained in the initial solution.
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[in Japanese]
Article type: Article
1957 Volume 12 Pages
39-
Published: 1957
Released on J-STAGE: December 22, 2017
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Kazunori TSUKIHARA, Tadao KUSUMOTO, Masakazu IZUMIYA
Article type: Article
1957 Volume 12 Pages
40-44
Published: 1957
Released on J-STAGE: December 22, 2017
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Using a whole jacketed rotary cylindrical pilot plant of 180 liters in capacity, the authors succeeded in culturing Eremothecium ahbyii on cereal germ obtaining 4-8 mg of riboflavin production per g of dried mash by 5 days culture and 8-10 mg by 8 days.
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Tokio KOBAYASHI, Shoichi SHIMIZU
Article type: Article
1957 Volume 12 Pages
44-47
Published: 1957
Released on J-STAGE: December 22, 2017
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The authors tried to purify arabinose solution by a system of Amberlite IRA-400 (-OH) and Amberlite IR-120 (-H), but it was undesirable as the sugar was partly decomposed by IRA-400 (-OH). However, the purification succeeded without decomposition when a system of Amberlite IR-120 (-H) and Amberlite IRA-400 (-CO_3) was used, and the yield of arabinose through the system was about 80 per cent.
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[in Japanese]
Article type: Article
1957 Volume 12 Pages
47-
Published: 1957
Released on J-STAGE: December 22, 2017
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Tokio KOBAYASHI
Article type: Article
1957 Volume 12 Pages
48-51
Published: 1957
Released on J-STAGE: December 22, 2017
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The author tried to analyze mathematically the intensity of the fluorescence of flavinadeninedinucleotide using Kavanagh's equation, and obtained the following conclusions : (1) Only in dilute solutions of flavinadeninedinucleotide less than 1 μg/ml, the intensity of fluorescence was proportional to the concentration, therefore the fluorometric determination of it should be done in this range.(2) Putting the intensity of fluorescence of 10 μg/ml solution at 100,that of 50 μg/ml was 149,and no further increase was found by increasing the concentration over this level.
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[in Japanese]
Article type: Article
1957 Volume 12 Pages
51-
Published: 1957
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1957 Volume 12 Pages
51-
Published: 1957
Released on J-STAGE: December 22, 2017
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Isao NAKAMURA
Article type: Article
1957 Volume 12 Pages
52-56
Published: 1957
Released on J-STAGE: December 22, 2017
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The prophylaxis and therapy of the rickets of albino rats were studied with different dosages of vitamin D (50,100 and 200 I.U. per week per os). The effect of vitamin D was estimated by alkaline phosphatase activities in the serum, bone, kidney and intestine. In the prophylactic experiment, it could be fund a parallelism between the doses of vitamin D and the alkaline phosphatase activities in the serum or the bone, especially in the latter the phosphatase activities were inversely proportional to the logarithms of the doses of vitamin D. But such relations could not be found in therapeutic experiments. In both experiments, the variation of the alkaline phosphatase activity of serum seemed to have no correlation with that in the kidney and intestine.
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[in Japanese]
Article type: Article
1957 Volume 12 Pages
56-
Published: 1957
Released on J-STAGE: December 22, 2017
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Seiichi TANIGUCHI
Article type: Article
1957 Volume 12 Pages
57-64
Published: 1957
Released on J-STAGE: December 22, 2017
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Pyridoxine + pyridoxamine, pyridoxal and 4-pyridoxic acid were determined in 24 hour urine after the administration of aureomycin, terramycin or homosulfamine for healthy rabbits. In animals which were given aureomycin or terramycin, both pyridoxine + pyridoxamine and pyridoxal were apparently decreased after their administration, and remarkable change was recognized in 4-pyridoxic acid excretion. In several animals, pyridoxic acid pronouncedly decreased immediately after the administration and maintained its level and in others, it once decreased then increased to normal level and after 10-13 days it again decreased. In anima1s which were given homosulfamine, no marked change was seen except a slight increase in pyridoxal excretion.
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Seiichi TANIGUCHI
Article type: Article
1957 Volume 12 Pages
65-72
Published: 1957
Released on J-STAGE: December 22, 2017
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Pyridoxine + pyridoxamine, pyridoxal and 4-pyridoxic acid were determined daily after the administration of INAH or its metansulfonate for normal children or rabbits. Administration of INAH caused a remarkable increase in pyridoxal excretion and a slight decrease in 4-pyridoxic acid excretion, whereas the administration of INAH-metansulfonate caused almost no change for the excretion of pyridoxine + pyridoxamine, pyridoxal and 4-pyridoxic acid.
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Kazuyuki MATSUBARA
Article type: Article
1957 Volume 12 Pages
73-79
Published: 1957
Released on J-STAGE: December 22, 2017
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Even though oral dose of thiamine exceeded 10 mg, the urinary excretion of the vitamin showed no further increase than when the dose was 10 mg. On the contrary, the fecal excretion of thiamine showed a noticeable increase as oral dose of the vitamin became higher, particularly when it exceeded 10 mg. The thiamine content of blood showed only a slight increase after oral administration of the vitamin even if a large amount of the vitamin was administered. On the other hand, after subcutaneous injection of thiamine, its blood content showed a remarkable increase proportionally to the quantity of administration, having a tendency to fall rapidly to the original level.
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[in Japanese]
Article type: Article
1957 Volume 12 Pages
79-
Published: 1957
Released on J-STAGE: December 22, 2017
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Kazuyuki MATSUBARA
Article type: Article
1957 Volume 12 Pages
80-89
Published: 1957
Released on J-STAGE: December 22, 2017
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After thiamine, TAD or TPD was infused into the intestinal canal, blood levels of each in ve. intestinales and a. femoralis were determined, and it was confirmed that both TAD and TPD were absorbed more rapidly than thiamine in the intestines. At the same time, the contents of thiamine and TAD or TPD in the intestinal contents were assayed after TAD or TPD was infused into the intestinal canal, and it was found that a considerable amount of either TAD or TPD was reduced to thiamine readily in intestinal canal.
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[in Japanese]
Article type: Article
1957 Volume 12 Pages
89-
Published: 1957
Released on J-STAGE: December 22, 2017
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Kazuyuki MATSUBARA
Article type: Article
1957 Volume 12 Pages
90-97
Published: 1957
Released on J-STAGE: December 22, 2017
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Oral administration of TAD or TPD increased the thiamine excretion in the urine more than oral administration of thiamine did. Intravenous administration of S-allyl-mercaptocysteine, S-propylmercaptocysteine, allyldisulfide or propyldisufide did not increase the thiamine excretion in the urine. Oral administration of any of these substances did not increase the urinary thiamine excretion. Intravenous administration of any of these substances combined with oral administration of thiamine did not increase the thiamine excretion in the urine above the level found in a single oral administraion of thiamine. When either of these substances was given orally together with thiamine, the urinary thiamine discharge became larger compared with that at a single oral administration of thiamine. The magnitude of increase was simiar to that when TAD or TPD was administered orally.
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[in Japanese]
Article type: Article
1957 Volume 12 Pages
97-
Published: 1957
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1957 Volume 12 Pages
97-
Published: 1957
Released on J-STAGE: December 22, 2017
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Kazuyuki MATSUBARA, Nobuo ODA, Norio HANIOKA
Article type: Article
1957 Volume 12 Pages
98-101
Published: 1957
Released on J-STAGE: December 22, 2017
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By carrying out Sawada's urinary reaction with children on varying doses of fructose, it was found that the result of this reaction depended on doses of fructose, and there was no relation between this reaction and blood level of thiamine or urinary urobilinogen reaction.
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Itaru UTONISHI
Article type: Article
1957 Volume 12 Pages
102-105
Published: 1957
Released on J-STAGE: December 22, 2017
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The pharmacological actions of diphosphopyridinenucleotide (DPN) on peripheral vessels, cardiac systole and blood pressure were studied. It was found that DPN induced a slight contraction of peripheral vessels, but inhibited cardiac systole and brought about a decrease of blood pressure. These actions were also observed when adenosine was used in place of DPN. Atropine and pyribenzamine had no influence on the actions of adenosine or DPN on cardiac systole and blood pressure.
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Toshio NIIMURA
Article type: Article
1957 Volume 12 Pages
106-114
Published: 1957
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1957 Volume 12 Pages
114-
Published: 1957
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese]
Article type: Article
1957 Volume 12 Pages
114-
Published: 1957
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1957 Volume 12 Pages
114-
Published: 1957
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese]
Article type: Article
1957 Volume 12 Pages
115-
Published: 1957
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese]
Article type: Article
1957 Volume 12 Pages
115-
Published: 1957
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese]
Article type: Article
1957 Volume 12 Pages
115-
Published: 1957
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese]
Article type: Article
1957 Volume 12 Pages
115-116
Published: 1957
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese]
Article type: Article
1957 Volume 12 Pages
116-
Published: 1957
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese]
Article type: Article
1957 Volume 12 Pages
116-
Published: 1957
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese]
Article type: Article
1957 Volume 12 Pages
116-117
Published: 1957
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese]
Article type: Article
1957 Volume 12 Pages
117-
Published: 1957
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1957 Volume 12 Pages
117-
Published: 1957
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese]
Article type: Article
1957 Volume 12 Pages
117-118
Published: 1957
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1957 Volume 12 Pages
118-
Published: 1957
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese]
Article type: Article
1957 Volume 12 Pages
119-
Published: 1957
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese]
Article type: Article
1957 Volume 12 Pages
119-
Published: 1957
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1957 Volume 12 Pages
119-
Published: 1957
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1957 Volume 12 Pages
119-120
Published: 1957
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1957 Volume 12 Pages
120-
Published: 1957
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1957 Volume 12 Pages
120-
Published: 1957
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1957 Volume 12 Pages
120-121
Published: 1957
Released on J-STAGE: December 22, 2017
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