Abstract
In view of recent progresses in the separation and purification of flavin compounds from various organisms and of discoveries of new flavin compounds, the existence of flavin compound such as flavinhypoxanthinedinucleotide (FHD) seems to be not impossible. The chemical preparation of FHD was achieved by the deamination of FAD using HNO_2. There was little difference in the physicochemical properties between FHD and FAD except their absorption spectra in which the shoulder build at 250-255 mμ was specific for FHD. The biological activity of FHD was examined with D-amino acid oxidase, but no remarkable activity was found. The enzymatic deamination of FAD by adenosine deaminase prepared from takadiastase was examined spectrophotometrically, and FHD was demonstrated from the reaction mixture on the paper chromatography.
