Abstract
The relative potency of thiothiamine to thiamine was again assayed by prophylactic test with rats and mice and by Lactobacillus fermenti assay and it was found to be about 1/100,1/50 and 1/20000,respectively. Thiothiamine showed no thiamine effect both in rice bird assay (0.4μM) and in rat bradycardia test (1.0μM). These results led to the conclution that thiothiamine had almost no thiamine activity and confirmed the findings in rat growth assay by Suhara et al. Discrepancy between the results of the previous and present reports was also discussed.
