Abstract
FAD was extracted with pyridine-methanol-water mixture from wet mycellia of Eremothecium ashbyii and uptaken on a column of Amberlite IRA-401-Cl. After washing the column with a dilute aqueous solution of sodium chloride, FAD was desorbed with a 10% sodium chloride solution, vacuum concentrated, extracted with phenol, adding ether, reextracted with water and precipitated with ethanol. In this method, FAD preparations of 86.5% purity were obtained in a yield of 49.1% from mycellia cultured in a fermentor of 2 tons in capacity.
