Abstract
FAD was adsorbed on several commercial active carbon powder, with the capacity of from 10 to 15% of their weight. It was eluted with basic solvent more easily than with acidic solvents. Among them, the optimum ratio of pyridine-water was 1/1 and that of lutidine-water was 4/6. Addition of a small amount of amines, quinolines or nitrobenzene to pyridine-water (1/1) facilitated elution, but it was more excellent with lutidine-water (4/6). FAD was adsorbed on active carbon and the recovery of 80% or more was obtained with 5 times elutions using lutidine-water (4/6) of 10 fold volume of carbon weight. No purifying effect was observed in the above process.
