Abstract
Methods were described for the extraction of the intracellular thiaminase by use of dry-ice. Two kinds of enzyme thus obtained from Bacillus thiaminolyticus and Clostridium thiaminolyticum, respectively were characterized based on the rate of migration in an electric field. It was of interest that the extracted thiaminase from B.thiaminolyticus had stronger activity on thiamine than that from Cl.thiaminolyticum while the latter was found much purer than the former.
