I have proved the existence of the thiaminase, in the supernatant of the precipitation test, by which the Cl.thiaminolyticum culture and its immune serum was mixed. By the ring test the disapperance of the thiaminase was observed according to the time lapse and the serum volume. In the mixed method, 73〜84% thiaminase disappeared after 2 hours. Thus the author have demonstrated the existence of anti-thiaminase in the immune sera.
