Influence of thiaminase activity was examined by the addition of immune serum against Clostridium thiaminoliticum culture. The experiment was carried on with the most suitable condition, i.e.pH 7.5,37℃, 30 minutes. The result shows by the Yoshikawa strain 57〜67% and by the Tomikawa strain 25〜45% decrease of the activity. This is also the evidence that anti-thiaminase exists in the immune serum.
