Abstract
A microbiological assay method of vitamins in the urine after oral dose of chloramphenicol was proposed. Chloramphenicol resistant Streptococcus faecalis R was used as test organism, because the inhibitory effect on the growth of Lactobacilli was not excluded by heat, acid or alkali treatment. The chloramphenicol resistant Stc. faecalis R was used, which was isolated by means of successive transfer in the medium containing graded amount of chloramphenicol. The maximum growth of this strain in the assay medium was one half of the parent strain, but the assay ranges of vitamins such as biotin, pantothenic, nicotinic and folic acid, were the same with the parent strain. The standard curves for these vitamins with resistant strain were not influenced even by the presence of about 200μg/ml of chloramphenicol. When vitamins in the urine after oral dose of chloramphenicol were assayed using resistant strain, good results were obtained.
