Abstract
This report relates to a new synthetic medium for B.thiaminolyticus. The improved Kimura's C medium for B.aneurinolyticus by Sakakibara and Katsumata was used as the basal medium and studies on S-, N-, C-, P-source and other growth factors were carried out in detail. For the growth and enzyme production of B.thiaminolyticus, thiamine was required as S-source and glutamic acid as N-source, but glycerol or glucose being able to be utilized as C-source was not useful because of acidifying the synthetic medium. On the contrary, glutamic acid was used not only as a N-source, but as C-source. Thus the composition of a new synthetic medium for B.thiaminolyticus was as follows : glutaminate 20g, asparagine 5g, citrate 5g, NaCl 1g, KH_2PO_4 1g, (NH_4)_2HPO_4 5g, MgCl_2・6H_2O 0.7g, FeCl_2・6H_2O 0.03g, thiamine 500γ, water 1,000ml.
