Abstract
The glutamate metabolism of Bacillus aneurinolyticus treated at a low temperature (about 3℃)was studied. Keeping the cells at the temperature caused decrease of the metabolic rate, especially the rate of glutamate or pyrrolidone carboxylate metabolism. And a parallel decrease of thiaminase II activity with that of the metabolic rate was also observed under such a condition. Studies were made on change of the metabolic rate of glutamate or other substrates at different growth phases, namely logarithmic phase and phases before and after the spore formation. The metabolic rate of glutamate or pyrrolidone carboxylate was found to change in parallel with thiaminase II activity, and the highest rate was observed in the resting cells before the spore formation. These results suggest that thiaminase II is concerned in the glutamate metabolism passing through pyrrolidone carboxylate.
