Abstract
Thiamine benzenesulfonate in the alkaline medium is instantly transformed into thiamine-anhydride, from which no thiochrome is produced. The mixture of thiamine and its benzenesulfonate is kept alkaline for 5 minutes, then thiamine only can be determined by the usual thiochrome method when it is acidified, while without alkaline treatment the two are determined by the thiochrome method. 7.1 mg of thiamine benzenesulfonate was given to rats intraperitoneally or orally and the excretion of thiamine and its benzenesulfonate in 24 hours' urine was estimated : The average value in intraperitoneal dosage was 0.22 mg of thiamine and 5.88 mg of its benzenesulfonate, and that in oral dosage was 0.07 mg of thiamine and 0.36 mg of its benzenesulfonate. Thiamine benzenesulfonate was identified in the urine by paper partition chromatogrphy, indicating that it was excreted per se in the urine without hydrolysis into thiamine.
