The orange-red color, formed by the reaction of ascorbic acid and alkali, was attempted to use as a spectrophotometric determination of ascorbic acid. 0.5% N-bromosuccinimide, 1.5% SnCl_2 and 10 N NaOH are added to the sample solution with 2% metaphosphoric acid containing about 0.5 mg/ml of ascorbic acid, and the color formed is mesured by Beckman spectrophotometer at 520mμ.
