Abstract
The color reaction of ascorbic acid by alkali was applied to the determination of ascorbic acid in plant tissues. Mixed solution of 0.01% thiourea, 1% metaphosphoric acid and 50% ethyl alcohol was found to be more effective for removing the protein and stabilizing the ascorbic acid, increasing the sensibility of the reaction than metaphosphoric acid alone. Ascorbic acid in vegetable tissues was able to determine in an extract containing 0.01 to 0.4 mg/ml of ascorbic acid. The result obtained by the method was almost corresponding to those by indophenol or dinitrophenylhydrazine methods.
