Abstract
Experiment are performed on whether any correlation exists between the incubation temperature and biosynthesis of riboflavin or carbohydrate metabolism in the cells of Ashbya gossypii. There is a remarkable difference in the riboflavin biosynthesis at different temperatures of submerged culture. When the temperature is raised from 27 to 37℃, the riboflavin formation decreases greatly. But the oxidation velocity of sugary substances, except pentoses, and the organic acids contained in TCA-cycle members by the resting cells are apparently greater at 37 than at 27℃. The inhibitory effects of NaF, CH_2ICOOH and streptomycin on the glucose and organic acids metabolism are also generally greater at higher temperatures. The RQ in the glucose oxidation at 37 and 27℃ is 1.31 and 1.0 respectively. From the results obtained, it may be concluded that A. gossypii only produces riboflavin at a temperature which is lower than the optimum for the carbohydrate metabolism.
