EFFECT OF THIAMINE AND ITS DERIVATIVES ON THE GROWTH OF HUMAN AMNION CELLS (JTC-3 CELLS) IN TISSUE CULTURE : (II) EFFECT OF HIGHLY CONCENTRATED THIAMINE DERIVATIVES ON THE GROWTH OF JTC-3 CELLS

Abstract

The effect of thiamine and its derivatives at high concentrations over optimal on the growth of JTC-3 cells in a synthetic medium was investigated. As the concentration of the thiamine derivatives rose to 10^2,10^3 and 10^4 times of the optimal, the growth of the cells was impaired proportionally. At 10^4 times concentration, all of the compounds showed the impairment, especially conspicuous in TPD and TATD. At the concentration of 10μg/ml, the growth impairment was observed in TPD, TOED and BTMP, especially in the former.