Abstract
Paperchromatographic separation of L-ascorbic and D-araboascorbic acids were carried out using HPO_3 treated filter paper and water saturated methyl-ethyl ketone employing ascending method. The filter paper, pretreated with 3% HPO_3 solution and developing at 25℃ was the most suitable condition for separating them. The moisture in stationary phase is quite important for the separation, and polyhydric alcohol and their derivative, such as ethylene glycol, glycerol, their chlorohydrines and ethers, can be used as partition liquid by adding to the filter paper. By increasing the concentration of glycerol in 3% HPO_3 solution, locations of the both compounds were varied without change of separating ratio (0.1 as Rf value). This technique may be effective for the separation of these substances from natural product containing some impurities having the same Rf values with those acids.
