Abstract
In recent publications, several studies relating to the separation and estimation of L-ascorbic and D-araboascorbic acids using acetonitrile・acetone・acetic acid・water system and descending method were reported. Separating ratio of the both compounds was only 0.05 in Rf values. The authors has reported to separate them using water saturated methyl-ethyl ketone and HPO_3-treated filter paper employing ascending method. In present paper, with the application of the new method, the both compounds were separated and were estimated by 2,4-dinitrophenylhydrazine method. The experimental results were treated by analysis of linear regression and 95% fiducial range of regression lines were determined. 95% fiducial limits were ±3-4 μg for both compounds at a range between 10 to 50 μg, and ±2-3μg for 4 to 10μg.
