Abstract
Studies on thiamine-protein complexes were again made by the equilibrium dialysis method. The dialysis method was not suitable for the purpose of this experiment because BTDS was scarcely soluble in water, but the results of the experiment were essentially similar to those in the previous reports in regard to the significance of SH groups of proteins in the complex formation. The amounts of complexes determined by this method were not varied with pH in the dialysis but varied with pH of reaction mixture for the complex formation. From these results, the electrostatic adsorption of thiamine derivatives on protein seemed to be not concerned in the complex formation.
