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Volume 26 (1962)
- Issue 6 Pages 399-
- Issue 5 Pages 329-
- Issue 4 Pages 241-
- Issue 3 Pages 179-
- Issue 2 Pages 93-
- Issue 1 Pages 1-
Volume 26, Issue 2
Displaying 1-24 of 24 articles from this issue
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Masakazu IZUMIYA, Tokio KOBAYASHIArticle type: Article
1962Volume 26Issue 2 Pages 93-102
Published: August 25, 1962
Released on J-STAGE: January 25, 2018
JOURNAL FREE ACCESSDownload PDF (1269K) -
Kiyohide SONEArticle type: Article
1962Volume 26Issue 2 Pages 103-110
Published: August 25, 1962
Released on J-STAGE: January 25, 2018
JOURNAL FREE ACCESSJudging from the results of previous reports, it is possible supposed that the inactivation of α-amylase by riboflavin in the light is due to the denaturation of enzyme protein. In order to ascertain this supposition, the characteristic changes of optical density, specific rotation and color reaction of enzyme protein are determined before and after the illumination in this paper. The inactivated enzyme protein shows not only remarkable increases of its optical density at 280 mμ and specific rotation but also marked changes in its specific color reactions which are peculiar to the aromatic amino acids, such as tyrosine and tryptophan. From these results it is deduced that the inactivation of α-amylase by riboflavin the light is attributable to the denaturation of enzyme protein, depending upon the irreversible changes of aromatic amino acids.View full abstractDownload PDF (1275K) -
Akira YAMADAArticle type: Article
1962Volume 26Issue 2 Pages 110-115
Published: August 25, 1962
Released on J-STAGE: January 25, 2018
JOURNAL FREE ACCESSThe author has already reported that urinary excretion of riboflavin increased by the administration of calcium salts to rabbits, while it decreased by calciferol. It was found in this experiment that when calcium salts were given to rabbits, the phosphatase activity of serum, liver, kidney, small intestine and muscle showed higher values compared with the control, but lower values when calciferol was administered with calcium salts. It may therefore be concluded that calcium salts and calciferol have an antagonistic action in regard to riboflavin excretion in urine.View full abstractDownload PDF (582K) -
Goichiro KATSUI, Reiko SAIGUSAArticle type: Article
1962Volume 26Issue 2 Pages 116-120
Published: August 25, 1962
Released on J-STAGE: January 25, 2018
JOURNAL FREE ACCESSA series of crystalline complexes of all-trans-vitamin A aldehyde were formed with some phenolic compounds, such as pyrocatecol or o-cresol. Every of these complexes is different from any of similar ones described in the claims of Eastman Kodak's patents, the former having been consisted of equi-moles of vitamin A aldehyde and phenolic compounds. The binding ability of the complexes obtained in this study was so loose that it was easily capable to separate into two components by paper chromatography of calcium paper chromatographic technique or even by washing their ether solution with alkali.View full abstractDownload PDF (672K) -
Goichro KATSUIArticle type: Article
1962Volume 26Issue 2 Pages 121-123
Published: August 25, 1962
Released on J-STAGE: January 25, 2018
JOURNAL FREE ACCESSVitamin A aldehyde was orally administered to mice, weighing about 60g, in a dose of 3mg per mouse per day for 8 days. It was demonstrated that thus administered vitamin A aldehyde was not absorbed in itself but converted into the ester-type before absorption, which was derived from its alcoholic type produced in the intestine. It was also noted that thus the absorbed vitamin A ester was finally stored in the liver.View full abstractDownload PDF (314K) -
Isamu UTSUMI, Kiyoshi HARADAArticle type: Article
1962Volume 26Issue 2 Pages 123-127
Published: August 25, 1962
Released on J-STAGE: January 25, 2018
JOURNAL FREE ACCESSBy the heat decomposition of O-benzoylthiamine disulfide in ethylene glycol, light yellow scaly crystals (I), mp. 196-198℃ (decomp.) and colorless needle crystals (II), mp. 156-157℃ were obtained. It was proved that the crystal I was O-benzoylthiochrome. The crystal II was an unknown fluorescent substance. The fluorescence intensity of O-benzoylthiochrome was compared with that of thiochrome in aqueous and iso-butanol solutions. In aqueous solution, the fluorescence intensity of O-benzoylthiochrome was about 80% of thiochrome, but the intensity in iso-butanol solution was identical in both O-benzoylthiochrome and thiochrome.View full abstractDownload PDF (612K) -
Isamu UTSUMI, Kiyoshi HARADA, Keiichi KOHNOArticle type: Article
1962Volume 26Issue 2 Pages 128-133
Published: August 25, 1962
Released on J-STAGE: January 25, 2018
JOURNAL FREE ACCESSThe interactions between O-benzoylthiamine disulfide (BTDS) or its related compounds and proteins were studied by the methods of paper chromatography and zone electrophoresis. Thiamine-protein complexes and free thiamines were produced by the reaction of BTDS or other symmetrical thiamine disulfides with denatured egg albumin or with serum albumin, but these complexes were not formed by the reaction with the denatured proteins with blocked SH groups, as well as, with native egg albumin. The complexes were not separated to their components by means of paper chromatography or zone electrophoresis, but thiamine was liberated by the reaction with sodium thiosulfate. Therefore, the complex formation was supposed to be concerned with the SH group of proteins and seemed to be caused by the disulfide interchange between the SH groups of proteins and the S-S groups of the thiamine derivatives.View full abstractDownload PDF (697K) -
Isamu UTSUMI, Kiyoshi HARADA, Keiichi KOHNO, Hiroko HIRAOArticle type: Article
1962Volume 26Issue 2 Pages 134-139
Published: August 25, 1962
Released on J-STAGE: January 25, 2018
JOURNAL FREE ACCESSIn the previous report, it was observed qualitatively that O-benzoylthiamine disulfide (BTDS) and other symmetrical thiamine disulfide were reduced to free thiamine and further some part of them was converted to protein-thiamine complexes by the reaction with denatured egg albumin or serum albumin. In this report, quantitative investigations of complex formation were attempted. The complexes were separated from the reaction mixture by means of metaphosphoric acid deproteinization and the combined thiamine was determined by the thiochrome method, after the liberation of thiamine from the complex by treating with sodium thiosulfate. Then, the reaction of BTDS or its related compounds with the SH group of protein and the effects of pH, temperature and time etc. on the reaction were investigatigated. As a result of the experiment, these effects on the complex formation were made clear and it was confirmed that the complex was protein-thiamine mixed disulfide which was produced by the reaction with the SH groups of protein.View full abstractDownload PDF (755K) -
Isamu UTSUMI, Kiyoshi HARADA, Keiichi KOHNO, Yohko KONDO, Hiroko HIRAOArticle type: Article
1962Volume 26Issue 2 Pages 140-143
Published: August 25, 1962
Released on J-STAGE: January 25, 2018
JOURNAL FREE ACCESSStudies on thiamine-protein complexes were again made by the equilibrium dialysis method. The dialysis method was not suitable for the purpose of this experiment because BTDS was scarcely soluble in water, but the results of the experiment were essentially similar to those in the previous reports in regard to the significance of SH groups of proteins in the complex formation. The amounts of complexes determined by this method were not varied with pH in the dialysis but varied with pH of reaction mixture for the complex formation. From these results, the electrostatic adsorption of thiamine derivatives on protein seemed to be not concerned in the complex formation.View full abstractDownload PDF (521K) -
Tadakatsu KATO, Shoichi SHIMIZUArticle type: Article
1962Volume 26Issue 2 Pages 144-149
Published: August 25, 1962
Released on J-STAGE: January 25, 2018
JOURNAL FREE ACCESSCrude preparation of cyanocobalamin, prepared from feed supplement for vitamin B_<12> fortification, was found to contain a large amount of alcohol-insoluble, red-coloured substance. The substance, tentatively designated as X-factor, could be easily separated from cyanocobalamin by chromatography on P-cellulose column or by electrophoresis on powdered-cellulose column. Microbiological activity of X-factor was about one % of that of cyanocobalamin for Escherichia coli 215,vitamin B_<12> requiring mutant. No difference was observed between X-factor and cobinamide in paper-electrophoretic mobility and in absorption spectrum. It was also found that cobalamin was biosynthesized by the addition of X-factor together with 5,6-dimethylbenzimidazole to growing culture of E. coli mutant. From these results, X-factor seems to be one of incomplete vitamin B_<12>-analogues, probably cobinamide or a compound similar to it. The above described chromatographic procedure for isolation of X-factor is thought to be very simple and useful to prepare the selected precursor for biosynthesis of cobalamins.View full abstractDownload PDF (665K) -
Tadakatsu KATO, Shoichi SHIMIZUArticle type: Article
1962Volume 26Issue 2 Pages 150-153
Published: August 25, 1962
Released on J-STAGE: January 25, 2018
JOURNAL FREE ACCESSIn previous paper, we could readily isolate a substance, designated as X-factor, from bacterial cell products for feed supplement use and provided some evidence suggesting that the substance was cobinamide or its closely related compound. The present work was undertaken to ascertain whether X-factor is available as precursor in biosynthesis of cobamides with Propionibacterium shermanii. For this purpose, the organism was cultivated for 5 days at 30℃ in media containing various amounts of X-factor and 5,6-dimethylbenzimidazole (DBI) and the cobamides contents of cells and supernatant were determined by microbiological assay. For example, the cells containing about 2.2μg of cobamides per ml of medium were obtained when 3.0μg of X-factor and 2.0μg of DBI were added per ml of medium. While, in the supernatant, less amounts of cobamides were found. The addition of much amounts of X-factor caused a striking increase in the yield of cobamides in the cells. On the contrary, only 0.2μg of cobamides were found to be produced without added precursors.View full abstractDownload PDF (514K) -
Shinichiro KAWASHIMAArticle type: Article
1962Volume 26Issue 2 Pages 154-160
Published: August 25, 1962
Released on J-STAGE: January 25, 2018
JOURNAL FREE ACCESSA titration method of tube assay for lipoic acid with Streptococcus faecalis 10C1 described by Wada et al. who introduced the Stokstad's assay media to a titration method was investigated. The composition and pH of the assay media were slightly modified, with which growth of the microbes were more accelerated and incubation time was reduced. A suitable standard response curve after 24 hours incubation was ranged from 0.5 to 5 mμg/ml. The lipoic acid contents in non-hydrolyzed natural materials were measured. Recoveries of added DL-lipoic acid were sufficient in urine samples, though those in blood and tissues were sometimes more than theoretical values. These values were compared with those measured turbidimetrically in the samples which were treated to remove some interfering substances.View full abstractDownload PDF (629K) -
Shohai CHANGArticle type: Article
1962Volume 26Issue 2 Pages 161-167
Published: August 25, 1962
Released on J-STAGE: January 25, 2018
JOURNAL FREE ACCESSSix healthy adults and children were performed with oral administration of a new thiamine decomposing bacillus. All subjects were proved to be afficted with thiaminase disease and it was worthy of noted that 3 adults of them complained remarkable gastrointestinal disturbances and beriberi-llike disorders. On the other hand, in those adults administered with B. thiaminolyticus, thiaminase and decrease of amounts of thiamine in feces took place temporally, but no marked complaints occured, and thiaminase disease could not be proved in experiments by intake with B. aneurinolyticus. According to the results of immunological investigations, reactions of agglutination and precipitation with thiamine decomposing bacilli in human blood serum can be utlized to detect thiaminase disease.View full abstractDownload PDF (897K) -
Shohai CHANGArticle type: Article
1962Volume 26Issue 2 Pages 167-171
Published: August 25, 1962
Released on J-STAGE: January 25, 2018
JOURNAL FREE ACCESSImmunological reaction in blood serum of 340 cases, including healthy subjects, patients and pregnant womens with a new aerobic thiamine decomposing bacillus was performed with the following result. The positive percentage of agglutination reaction with a new thiamine decomposing bacillus and B. thiaminolyticus was 77% and 30%. No positive agglutination reaction with B. aneurinolyticus was seen. Therefore a new bacillus seemed to be widely distributed in human intestine and the most important bacterium resulting in thiaminase disease. According to many experimental results as previously reported and report in the present paper, a new bacillus can be determined perfectly to belong to a new species of Genus Bacillus different from B. thiaminolyticus in various points of view.View full abstractDownload PDF (734K) -
[in Japanese], [in Japanese]Article type: Article
1962Volume 26Issue 2 Pages 172-
Published: August 25, 1962
Released on J-STAGE: January 25, 2018
JOURNAL FREE ACCESSDownload PDF (169K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1962Volume 26Issue 2 Pages 172-173
Published: August 25, 1962
Released on J-STAGE: January 25, 2018
JOURNAL FREE ACCESSDownload PDF (325K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese]Article type: Article
1962Volume 26Issue 2 Pages 173-
Published: August 25, 1962
Released on J-STAGE: January 25, 2018
JOURNAL FREE ACCESSDownload PDF (208K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1962Volume 26Issue 2 Pages 173-
Published: August 25, 1962
Released on J-STAGE: January 25, 2018
JOURNAL FREE ACCESSDownload PDF (208K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1962Volume 26Issue 2 Pages 173-174
Published: August 25, 1962
Released on J-STAGE: January 25, 2018
JOURNAL FREE ACCESSDownload PDF (346K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1962Volume 26Issue 2 Pages 174-
Published: August 25, 1962
Released on J-STAGE: January 25, 2018
JOURNAL FREE ACCESSDownload PDF (190K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...Article type: Article
1962Volume 26Issue 2 Pages 174-
Published: August 25, 1962
Released on J-STAGE: January 25, 2018
JOURNAL FREE ACCESSDownload PDF (190K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1962Volume 26Issue 2 Pages 174-
Published: August 25, 1962
Released on J-STAGE: January 25, 2018
JOURNAL FREE ACCESSDownload PDF (190K) -
[in Japanese], [in Japanese]Article type: Article
1962Volume 26Issue 2 Pages 174-175
Published: August 25, 1962
Released on J-STAGE: January 25, 2018
JOURNAL FREE ACCESSDownload PDF (333K) -
[in Japanese], [in Japanese]Article type: Article
1962Volume 26Issue 2 Pages 175-
Published: August 25, 1962
Released on J-STAGE: January 25, 2018
JOURNAL FREE ACCESSDownload PDF (192K)
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