Abstract
Gas chromatography was applied to the separation of ten fat-soluble vitamins and their derivatives under several operating conditions. These fat-soluble vitamins and their derivatives could be classified to the three groups by the column temperature of gas chromatograph as follows : i) column temp. 200〜220℃ (vitamin D_2,D_3,E-acetate, K_1), ii) column temp. 150〜180℃. (vitamin A-alcohol, -acetate, -palmitate, K_4-diacetate), iii) column temp. below 130℃ (vitamin K_3). The collected fractions of vitamin A-alcohol and-acetate were studied by IR and UV spectra and thin layer chromatography. The results of these experiments suggested that vitamin A-alcohol and-acetate had converted to their anhydro-forms.
