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Volume 27 (1963)
- Issue 6 Pages 423-
- Issue 5 Pages 331-
- Issue 4 Pages 251-
- Issue 3 Pages 163-
- Issue 2 Pages 97-
- Issue 1 Pages 1-
Volume 27, Issue 5
Displaying 1-31 of 31 articles from this issue
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[in Japanese]Article type: Article
1963Volume 27Issue 5 Pages 331-332
Published: May 25, 1963
Released on J-STAGE: January 27, 2018
JOURNAL FREE ACCESSDownload PDF (315K) -
[in Japanese]Article type: Article
1963Volume 27Issue 5 Pages 333-335
Published: May 25, 1963
Released on J-STAGE: January 27, 2018
JOURNAL FREE ACCESSDownload PDF (494K) -
[in Japanese]Article type: Article
1963Volume 27Issue 5 Pages 336-341
Published: May 25, 1963
Released on J-STAGE: January 27, 2018
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Ryoji HAYASHI, Zensaku YOSHII, Tomoyasu HARADA, Kimitoshi NIGOTA, Yoko ...Article type: Article
1963Volume 27Issue 5 Pages 342-344
Published: May 25, 1963
Released on J-STAGE: January 27, 2018
JOURNAL FREE ACCESSThiaminase I was detected in culture supernatant fluid of 42 strains out of 44 of Cl. sporogenes and 9 organisms out of 10 which related closely to Cl. sporogenes. On the basis of this experimental result the authors concluded that thiaminase I is widely distributed among Cl. sporogenes.View full abstractDownload PDF (350K) -
Ryoji HAYASHI, Zensaku YOSHII, Tomoyasu HARADA, Kimitoshi NIGOTA, Yoko ...Article type: Article
1963Volume 27Issue 5 Pages 345-348
Published: May 25, 1963
Released on J-STAGE: January 27, 2018
JOURNAL FREE ACCESSThiaminase activity of culture supernatant fluid of clostridia which were received from many institutes was examined. The number of thiaminase positive cultures was as follows : 1) 2 strains out of 2 of Cl. aerofoetidum, 2) 6 out of 21 of Cl. bifermentans, 3) 3 out of 5 labeled as an organism closely related to Cl. bifermentans, 4) 17 out of 27 of Cl. botulinum, 5) 2 out of 15 of Cl. chauvoei, 6) 2 out of 2 of Cl. saprogenes, 7) 16 out of 20 of Cl. sporogenes, 8) 1 out of 3 of Cl. tetanomorphum and 9) 6 out of 14 labeled as an organism which could not be identified according to Bergey's Manual. Thiaminase of any of these cultures belonged to thiaminase I. So, the authors concluded that the production of thiaminase I is one of common properties of many species of sporeforming anaerobic bacteria and that Cl. thiaminolyticum should be reexamined taxonomically.View full abstractDownload PDF (469K) -
Teruo NINOMIYA, Kazuyuki KIDOKORO, Masaaki HORIGUCHI, Nobuyuki HIGOSAK ...Article type: Article
1963Volume 27Issue 5 Pages 349-354
Published: May 25, 1963
Released on J-STAGE: January 27, 2018
JOURNAL FREE ACCESSGas chromatography was applied to the separation of ten fat-soluble vitamins and their derivatives under several operating conditions. These fat-soluble vitamins and their derivatives could be classified to the three groups by the column temperature of gas chromatograph as follows : i) column temp. 200〜220℃ (vitamin D_2,D_3,E-acetate, K_1), ii) column temp. 150〜180℃. (vitamin A-alcohol, -acetate, -palmitate, K_4-diacetate), iii) column temp. below 130℃ (vitamin K_3). The collected fractions of vitamin A-alcohol and-acetate were studied by IR and UV spectra and thin layer chromatography. The results of these experiments suggested that vitamin A-alcohol and-acetate had converted to their anhydro-forms.View full abstractDownload PDF (570K) -
Toshio NIIMURA, Takao SUZUKI, Yoshikazu SAHASHIArticle type: Article
1963Volume 27Issue 5 Pages 355-361
Published: May 25, 1963
Released on J-STAGE: January 27, 2018
JOURNAL FREE ACCESSSulfur source in the biosynthesis of biotin from desthiobietin by washed cells of Saccharomyces cerevisiae which were deficient in biotin, or in biotin and sulfur was investigated, and it was elucidated that methionine acted an important role. In the case of biotin-and sulfur-deficient cells, biotin was produced in better yield in the procedure carried out by the addition of glucose and desthiobiotin after preincubation with glucose and sulfur compounds, than the procedure carried out by the addition of glucose, desthiobiotin and sulfur compounds without preincubation. The most effective sulfur compounds were methionine sulfoxide and methionine, followed by Na_2SO_4,Na_2SO_3 and Na_2S. Cysteine, cystine and methionine sulfone were less effective. In the case of Na_2SO_4,the further addition of glucose in combination with desthiobiotin was necessary after preincubation, but not necessary in the case of methionine. The biosynthesis of biotin by biotin-deficient cells was inhibited by ethionine and reversed only by methionine.View full abstractDownload PDF (729K) -
Chikataro KAWASAKI, Chisae YAMADAArticle type: Article
1963Volume 27Issue 5 Pages 362-365
Published: May 25, 1963
Released on J-STAGE: January 27, 2018
JOURNAL FREE ACCESSThe growth-stimulation of Lactobacillus fermenti or Kloeckera apiculata by the aseptic addition of such S-alkyl-thiamines as methyl-(I), ethyl-(II), benzyl-(III), 3,4,5-trimethoxybenzyl-(IV) and triphehylmethyl-(V) ether of thiamine or phosphates of (IV) and (V) to the thiamine-free broth, was investigated : (V) or its phosphate was proved to show one-tenth the growth-stimulation of thiamine both on Lactobacillus and Kloeckera, while the other compounds were shown to be practically inert. (III) or (IV) was proved to have one-thousandth the potency of thiamine on Lactobacillus assay and the others were far less potent than these compounds. Bioautography of (V) using L. fermenti revealed that its microbiological activity did not originate in the thiamine contaminated. The addition of (I), (II) or (III) each at the concentration of 1μg to 10 mg to the broth containing 0.05 μg of thiamine, resulted in noninhibition to the growth of both Lactobacillus and Kloeckera. S-Alkyl-thiamines, therefore, seem not to compete with thiamine.View full abstractDownload PDF (380K) -
Chikataro KAWASAKI, Chisae YAMADAArticle type: Article
1963Volume 27Issue 5 Pages 366-368
Published: May 25, 1963
Released on J-STAGE: January 27, 2018
JOURNAL FREE ACCESSS-Methyl-(I), S-ethyl-(II), S-benzyl-(III), S-3,4,5-trimethoxybenzyl-(IV) and S-triphenyl-methyl-(V) thiamines were shown to be thiochrome negative by the cyanogen bromide-NaOH procedure immediately after preparation of their 10^<-5>M solutions in 1% alcohol. A 10^<-5>M solution of (V) in 1% alcohol was easily hydrolyzed into thiamine at 37℃ to an extent of 28% yield in 24 hours, although the other compounds except (V) were proved to be stable. If (V) is incubated at the concentration of 1/4×10^<-5>M in the broth for L. fermenti or Kl. apiculata without innoculation, it is converted to thiamine to an extent of 57% or 44% yield in 24 hours. The stability of (V) is also influenced by pH, turning more unstable at lower values of pH. Complete conversion of (V) into thiamine, when incubated at 55〜60℃ for 30 minutes, was observed at pH 1 and at the presence of cysteine, the hydrolysis was rather inhibited.View full abstractDownload PDF (369K) -
Iwao YAMASAKIArticle type: Article
1963Volume 27Issue 5 Pages 369-372
Published: May 25, 1963
Released on J-STAGE: January 27, 2018
JOURNAL FREE ACCESSThe soluble fraction of the homogenates of the liver, kidneys, small intestines, heart, lungs, spleen and brain in the rat was obtained by Spinco ultracentrifuge (100,000×g, 60min.), and the phosphorylating activity of thiamine in the supernatant was studied. The reaction mixture contained thiamine (5μg), ATP (10^<-3>M) as a phosphate donor, Mg^<2+>(10^<-3>M) and the fraction. The activity was found in the liver, kidneys and intestines but not in the spleen, heart, lungs and brain.View full abstractDownload PDF (514K) -
Iwao YAMASAKIArticle type: Article
1963Volume 27Issue 5 Pages 373-377
Published: May 25, 1963
Released on J-STAGE: January 27, 2018
JOURNAL FREE ACCESSThiaminokinase from rat liver was purified about 5 folds compared with liver supernatant by Mano's method. It was found that esterfied thiamine product derived from thiamine was thiamine diphosphate with bioautography using Escherichia coli mutants. It was found that the activity was not present in the brain of the rat and cat.View full abstractDownload PDF (577K) -
Iwao YAMASAKIArticle type: Article
1963Volume 27Issue 5 Pages 378-383
Published: May 25, 1963
Released on J-STAGE: January 27, 2018
JOURNAL FREE ACCESSThe phosphorylating and dephosphorylating activities of thiamine in the mitochondria and supernatant of the rat liver, orally administered with a large or small quantities of Aureomycin were discussed. The phosphorylating activity of thiamine in the mitochondria and supernatant did not change by the quantity of Aureomycin. The dephosphorylating activity of esterified thiamine in the liver supernatant did not change in the second week, but the activity increased slightly in the fourth week of the Aureomycin administration.View full abstractDownload PDF (742K) -
Iwao YAMASAKIArticle type: Article
1963Volume 27Issue 5 Pages 384-387
Published: May 25, 1963
Released on J-STAGE: January 27, 2018
JOURNAL FREE ACCESSAddition of 20 or 200 μg/ml of Aureomycin or Achromycin to the supernatant of rat liver homogenate caused no change in phosphorylating activity of thiamine.View full abstractDownload PDF (483K) -
Iwao YAMASAKIArticle type: Article
1963Volume 27Issue 5 Pages 388-392
Published: May 25, 1963
Released on J-STAGE: January 27, 2018
JOURNAL FREE ACCESSThe phosphorylating and dephosphorylating activities of thiamine in the rat liver with carbon tetrachloride poisoning were discussed. The phosphorylating activity increased slightly in the supernatant and the mitochondria, but the activity did not change in the nuclei. The dephosphorylating activity of esterfied thiamine increased in the supernatant, mitochondria and administnuclei. These results suggest that the decrease of thiamine content of the rat liver by the ration of carbon tetrachloride is owing to an increasing dephosphorylation rather than a decreasing phosphorylation.View full abstractDownload PDF (586K) -
Masao OHARA, Kiyoshi MIZUTANI, Yoshio DEGUCHI, Kazuo FUJISAKI, Shozabu ...Article type: Article
1963Volume 27Issue 5 Pages 393-401
Published: May 25, 1963
Released on J-STAGE: January 27, 2018
JOURNAL FREE ACCESSInvestigation was made on the physicochemical properties and quantitative determination of TATD. TATD is a white odorless, crystalline substance, practically insoluble in water, but TATD hydrochloride has a bitter taste and is very soluble in water. The ultraviolet absorption spectrum of its aqueous solution is variable with changes in the pH. Its isosbestic points are at 240,275 and 303 mμ. An aqueous solution of TATD is stable to heat in acidic enviroments. The powder of TATD is very stable even when mixed with lactose or precipitated calcium carbonate. TATD is reduced by sodium thiosulfate, cysteine, sodium hydrosulfite and zinc powder. The optimum condition of reduction was studied. TATD (1〜200μg) was reduced quantitatively with 10mg of sodium thiosulfate in a pH of 4〜5 at 40〜60℃ for 15 minutes. Thiamine thus produced was determined by the thiochome method. S-Acetyl radical was also determined by the formation of acetohydroxamic acid according to Lipmann and Tuttle.View full abstractDownload PDF (898K) -
Fumio HONDA, Hiroko HOSHINOArticle type: Article
1963Volume 27Issue 5 Pages 402-405
Published: May 25, 1963
Released on J-STAGE: January 27, 2018
JOURNAL FREE ACCESSDecline in growth in male Wistar rats receiving a synthetic thiamine-deficient diet was effectively protected by the daily administration of TATD in dosage equivalent to 5 or 100μg of thiamine hydrochloride per animal as has been observed in similar administration of thiamine. Growth promoting activity of TATD in Streptococcus faecalis 10C1 was approximately equivalent to that of lipoic acid on the basis of a mole. However, since it was observed that disulfide linkage of TATD was split to liberate thiamine under the condition of heat sterilization or incubation in a culture medium, the response of bacterial cells may not be to TATD molecule but to lipoate derivative released from TATD.View full abstractDownload PDF (512K) -
STUDIES ON THIAMINE-8-(METHYL 6-ACETYLDIHYDROTHIOCTATE) DISULFIDE : (III) METABOLISM IN RABBIT BLOODFumio HONDA, Hiroko HOSHINOArticle type: Article
1963Volume 27Issue 5 Pages 406-410
Published: May 25, 1963
Released on J-STAGE: January 27, 2018
JOURNAL FREE ACCESSOn incubation of TATD with heparinized blood of a rabbit, thiamine liberation was observed by paper partition chromatography. Liberation of another counterpart, the lipoate moiety, was investigated bioautographically after the paper electrophoresis. Mobility of an active band which was detected after the incubation was identical with that of lipoic acid. These results show reductive splitting of disulfide linkage in TATD and hydrolysis of ester bond of lipoate moiety by the blood preparation. TATD reducing activity of rabbit blood was present predominantly in blood cell and the apparent ratio of the activity of blood cell to plasma was calculated to be about 12. The hydrolyzing activity of rabbit blood to methyl dihydrolipoate was found in both of blood cell and plasma in about same degree. Presence of deacetylation activity for S-acetyl derivative of dihydrolipoic acid in rabbit blood preparation was ascertained by means of the method of Lipmann and Tuttle. These results indicate that TATD is restored to its original components, thiamine and dihydrolipoic acid (or lipoic acid), in rabbit blood.View full abstractDownload PDF (594K) -
Fumio HONDA, Hiroko HOSHINOArticle type: Article
1963Volume 27Issue 5 Pages 411-415
Published: May 25, 1963
Released on J-STAGE: January 27, 2018
JOURNAL FREE ACCESSBlood thiamine level elevated by the intravenous injection of TATD was higher and maintained longer in blood than when thiamine was administered. This was ascribed to prominent distribution and to a higher accumulation of free thiamine in blood cells, in contrast with thiamine administration. This characteristic was also observed in a washed blood cell suspension system in vitro. In spite of such an unique behaviour of thiamine moiety of TATD it was found that lipoate moiety was not accumulated in blood cell. Lipoic acid itself was not also concentrated in blood cell. The difference between behaviour of thiamine moiety and of lipoate moiety of TATD towards blood cell is discussed.View full abstractDownload PDF (652K) -
[in Japanese], [in Japanese]Article type: Article
1963Volume 27Issue 5 Pages 416-
Published: May 25, 1963
Released on J-STAGE: January 27, 2018
JOURNAL FREE ACCESSDownload PDF (165K) -
[in Japanese], [in Japanese]Article type: Article
1963Volume 27Issue 5 Pages 416-
Published: May 25, 1963
Released on J-STAGE: January 27, 2018
JOURNAL FREE ACCESSDownload PDF (165K) -
[in Japanese], [in Japanese]Article type: Article
1963Volume 27Issue 5 Pages 416-417
Published: May 25, 1963
Released on J-STAGE: January 27, 2018
JOURNAL FREE ACCESSDownload PDF (312K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese]Article type: Article
1963Volume 27Issue 5 Pages 417-
Published: May 25, 1963
Released on J-STAGE: January 27, 2018
JOURNAL FREE ACCESSDownload PDF (196K) -
[in Japanese], [in Japanese]Article type: Article
1963Volume 27Issue 5 Pages 417-
Published: May 25, 1963
Released on J-STAGE: January 27, 2018
JOURNAL FREE ACCESSDownload PDF (196K) -
[in Japanese], [in Japanese]Article type: Article
1963Volume 27Issue 5 Pages 417-
Published: May 25, 1963
Released on J-STAGE: January 27, 2018
JOURNAL FREE ACCESSDownload PDF (196K) -
[in Japanese], [in Japanese]Article type: Article
1963Volume 27Issue 5 Pages 417-418
Published: May 25, 1963
Released on J-STAGE: January 27, 2018
JOURNAL FREE ACCESSDownload PDF (347K) -
[in Japanese], [in Japanese]Article type: Article
1963Volume 27Issue 5 Pages 418-
Published: May 25, 1963
Released on J-STAGE: January 27, 2018
JOURNAL FREE ACCESSDownload PDF (201K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1963Volume 27Issue 5 Pages 418-
Published: May 25, 1963
Released on J-STAGE: January 27, 2018
JOURNAL FREE ACCESSDownload PDF (201K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1963Volume 27Issue 5 Pages 418-
Published: May 25, 1963
Released on J-STAGE: January 27, 2018
JOURNAL FREE ACCESSDownload PDF (201K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1963Volume 27Issue 5 Pages 418-419
Published: May 25, 1963
Released on J-STAGE: January 27, 2018
JOURNAL FREE ACCESSDownload PDF (334K) -
[in Japanese], [in Japanese]Article type: Article
1963Volume 27Issue 5 Pages 419-
Published: May 25, 1963
Released on J-STAGE: January 27, 2018
JOURNAL FREE ACCESSDownload PDF (185K) -
[in Japanese], [in Japanese]Article type: Article
1963Volume 27Issue 5 Pages 419-
Published: May 25, 1963
Released on J-STAGE: January 27, 2018
JOURNAL FREE ACCESSDownload PDF (185K)
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