BIOSYNTHESIS OF BIOTIN FROM DL-DESTHIOBIOTIN BY WASHED CELLS OF SACCHAROMYCES CEREVISIAE : (III) SULFUR SOURCE IN THE BIOSYNTHESIS OF BIOTIN : (2) DISTRIBUTION OF ^<35>S IN THE CELLS INCUBATED WITH ^<35>S-LABELED SULFURIC ACID

Abstract

Distribution of ^<35>S in the both biotin and sulfur deficient cells of Saccharomyces cerevisiae which have been incubated in the reaction mixture containing H_2S^*O_4 and glucose for 1 hour, was investigated by autoradiography. The countered relative amounts (cpm) of ^<35>S were 1054 in methionine, 340 in methionine sulfoxide, 120 in cysteic acid and 44 in cystine, and several other compounds showed less radioactivity. The incorporated ^<35>S was practically restricted in methionine and methionine sulfoxide, and was not found in the original sulfate form.