Abstract
Penetration of thiamine propyldisulfide (TPD) or thiothiamine (SB_1) into bovine erythrocytes was investigated upon their suspension in physiological saline solution. TPD was quickly absorbed by erythrocytes and the thiamine level in erythrocytes was either kept unchanged for 24 hours or not washed out by repeated shaking with saline solutions. SB_1 was found almost equally in erythrocytes and the supernatant, after incubation for one hour. SB_1 in erythrocytes was partly washed out by shaking with saline solution, as SB_1 existed as itself in the erythrocytes. TPD, when penetrated into blood cells, was reduced to thiamine which was no more washed out by saline solution. Only one spot of thiamine was demonstrated in hemolysate of the erythrocytes by means of paperchromatography.
