Abstract
S-Methylmethionine sulfonium chloride (MMSC) itself had no methionine activity on L. arabinosus, but showed the activity only when homocysteine was added. This function was charactiristic of MMSC and homocysteine, and choline, betaine or carnitine did not replace MMSC, nor did thioglycolate and cysteine. From these results, the microbioassay of DL-MMSC ranging 10 to 50μg was established. L. arabinosus was used as test microorganism and 200μg/tube of DL-homocysteine was added. The stabilities of MMSC to acid, alkali and heat were observed. MMSC was stable at room temperature. It was, however, labile at 100℃ except in acidic solution. The determination of MMSC in commercial preparations was tried.
