1963 Volume 28 Issue 6 Pages 488-500
Measurement of radioactivity in the organs, urine and feces in men and experimental aminals after oral or intramuscular injection of ^<35>S-labelled thiamine hydrochloride or thiamine propyldisulfide, revealed that the amount of urinary radioactive substances other than thiamine was one third of the total radioactivity. Main fraction among nine radioactive substances in urine was isolated by adsorbing on Amberlite IR-410 resin. This substance, having Rf value 0.7 with water- saturated n-butanol, did not have fluorescence under ultraviolet light, and was negative for thiochrome test, indicated not having thiamine molecule by bioautography, and did not behave like any known derivatives or decomposition products of thiamine.