RADIOACTIVE SUBSTANCES ELIMINATED IN URINE, FOLLOWING INTRAVENOUS INJECTION OF RADIOACTIVE LIPOIC ACID : (II) SEPARATION OF PRINCIPAL METABOLITES OF LIPOIC ACID IN URINE BY PHENOL-EXTRACTION

Abstract

It was reported in previous paper that eleven ratioactive substances were detected in the urine of normal human subjects following intravenous injection of lipoic acid labelled with S^<35>, including a small amount of free lipoic acid. Further investigation was done to separate the main metabolic products in the urine by ethylacetate and phenol extraction and separated into fraction I to IV using Sephadex column. Fraction I and IV responded for Streptococcus faecalis R and thought to be lipoic acid derivatives.