VITAMINS

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Volume 29, Issue 5

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[title in Japanese]

[in Japanese]

Article type: Article
1964 Volume 29 Issue 5 Pages 309-
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_309

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[title in Japanese]

[in Japanese]

Article type: Article
1964 Volume 29 Issue 5 Pages 310-322
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_310

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[title in Japanese]

[in Japanese]

Article type: Article
1964 Volume 29 Issue 5 Pages 323-330
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_323

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[title in Japanese]

[in Japanese]

Article type: Article
1964 Volume 29 Issue 5 Pages 331-335
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_331

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[title in Japanese]

[in Japanese]

Article type: Article
1964 Volume 29 Issue 5 Pages 336-341
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_336

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[title in Japanese]

[in Japanese]

Article type: Article
1964 Volume 29 Issue 5 Pages 342-344
Published: May 25, 1964
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DOIhttps://doi.org/10.20632/vso.29.5_342

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[title in Japanese]

[in Japanese]

Article type: Article
1964 Volume 29 Issue 5 Pages 345-347
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_345

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[title in Japanese]

[in Japanese]

Article type: Article
1964 Volume 29 Issue 5 Pages 348-352
Published: May 25, 1964
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DOIhttps://doi.org/10.20632/vso.29.5_348

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[title in Japanese]

[in Japanese]

Article type: Article
1964 Volume 29 Issue 5 Pages 353-355
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_353

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[title in Japanese]

[in Japanese]

Article type: Article
1964 Volume 29 Issue 5 Pages 356-359
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_356

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[title in Japanese]

[in Japanese]

Article type: Article
1964 Volume 29 Issue 5 Pages 360-363
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_360

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[title in Japanese]

[in Japanese]

Article type: Article
1964 Volume 29 Issue 5 Pages 364-367
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_364

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[title in Japanese]

[in Japanese]

Article type: Article
1964 Volume 29 Issue 5 Pages 368-376
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_368

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[title in Japanese]

[in Japanese]

Article type: Article
1964 Volume 29 Issue 5 Pages 377-380
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_377

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[title in Japanese]

[in Japanese]

Article type: Article
1964 Volume 29 Issue 5 Pages 381-383
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_381

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[title in Japanese]

[in Japanese]

Article type: Article
1964 Volume 29 Issue 5 Pages 384-389
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_384

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[title in Japanese]

[in Japanese]

Article type: Article
1964 Volume 29 Issue 5 Pages 390-396
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_390

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[title in Japanese]

[in Japanese]

Article type: Article
1964 Volume 29 Issue 5 Pages 397-401
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_397

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[title in Japanese]

[in Japanese]

Article type: Article
1964 Volume 29 Issue 5 Pages 402-403
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_402

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[title in Japanese]

[in Japanese]

Article type: Article
1964 Volume 29 Issue 5 Pages 404-409
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_404

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[title in Japanese]

[in Japanese]

Article type: Article
1964 Volume 29 Issue 5 Pages 410-416
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_410

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[title in Japanese]

[in Japanese]

Article type: Article
1964 Volume 29 Issue 5 Pages 417-419
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_417

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[title in Japanese]

[in Japanese]

Article type: Article
1964 Volume 29 Issue 5 Pages 420-422
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_420

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[title in Japanese]

[in Japanese]

Article type: Article
1964 Volume 29 Issue 5 Pages 423-424
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_423

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[title in Japanese]

[in Japanese]

Article type: Article
1964 Volume 29 Issue 5 Pages 425-426
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_425

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[title in Japanese]

[in Japanese]

Article type: Article
1964 Volume 29 Issue 5 Pages 427-428
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_427

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[title in Japanese]

[in Japanese]

Article type: Article
1964 Volume 29 Issue 5 Pages 429-430
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_429

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[title in Japanese]

[in Japanese]

Article type: Article
1964 Volume 29 Issue 5 Pages 431-433
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_431

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[title in Japanese]

[in Japanese]

Article type: Article
1964 Volume 29 Issue 5 Pages 434-
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_434

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[title in Japanese]

Article type: Appendix
1964 Volume 29 Issue 5 Pages 435-436
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_435

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STUDIES ON THE SYNTHESIS OF DINUCLEOTIDES : (II) SYNTHESIS OF FAD AND ITS ACYL-DERIVATIVES

Toshio KURODA

Article type: Article
1964 Volume 29 Issue 5 Pages 437-444
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_437

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Flavin adenine dinucleotide was synthesized in a yield of 52% from adenosine-5-phosphoromorpholidate (Ia) and riboflavin-5-phosphate-trioctylammonium using dimethylformamide as the solvent. By a similar procedure 2', 3', 4'-triacylriboflavin adenosine diphosphate (acyl radical CH_3CO or C_2H_5CO) was synthesized in a yield of 17 and 35% from 2', 3', 4'-triacylriboflavin-5-phophate and Ia, respectively. The products were purified by chromatography on ion exchange resin column.

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NUTRITIONAL STUDIES ON THE SCHOOL LUNCH PROGRAM : (II) THIAMINE AND RIBOFLAVIN CONTENTS IN THE BREADS AND FLOURS

Hyozo KAWAKITA, Keiiti OSIBA, Kunio OKAZAKI

Article type: Article
1964 Volume 29 Issue 5 Pages 445-449
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_445

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Thiamine and riboflavin contents of the 156 samples of the bread in 1960 and the 89 samples in 1961 were determined. The mean contents of thiamine (0.29〜0.31mg%) and that of riboflavin (0.20〜0.23mg%) found in them were seemed to be lower than the standard. The results of the determination of thiamine and riboflavin in the 64 samples of the semistrong flour agreeded nearly to the standard but the results of them in the 81 samples of the strong flour were slightly lower than the standard. The correlation with riboflavin contents in the bread and the flour was significant (r=0.4778) but that with thiamine contents in them was insignificant (r=0.0938).

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STUDIES ON THIOTHIAMINE : (IX) ISOLATION OF A PYRIMIDINE DERIVATIVE FROM THE URINE OF RATS AFTER INJECTION OF THIOTHIAMINE

Nobuko IRITANI

Article type: Article
1964 Volume 29 Issue 5 Pages 450-453
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_450

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A pyrimidine derivative from the urine was isolated as a metabolite of thiothiamine, when thiothiamine was given parenterally to rats. The urine was extracted with ether and then with butanol, acid and alkaline, respectively. Each extract was concentrated and was extracted with water. The water-soluble fraction separated from an oily residue, showed the presence of four Dragendorff-positive spots on paper chromatography. A pyrimidine derivative was separated as a picrate and after reconversion to hydrochloride of the free base, needles of mp. 260℃ (decomp.) were isolated by using cellulose column chromatography. It was identified as 2-methyl-4-amino-5-aminomethylpyrimidine hydrochloride by mixed melting point, UV spectrum or Rf value and also as picrate (mp. 228℃) which coincided with an authentic sample both in mixed melting point and in elementary analysis.

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STUDIES ON THIOTHIAMINE : (X) COMPARISON OF METABOLISMS OF THIOTHIAMINE AND ITS DERIVATIVES IN RATS

Nobuko IRITANI

Article type: Article
1964 Volume 29 Issue 5 Pages 454-458
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_454

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When 25 or 50 mg of thiothiamine was given parenterally to rats, 2-methyl-4-amino-5-aminomethylpyrimidine (I) was excreted in the 24 hour urine in the amount of less than 0.1 or 0.36 mg while excretion of sulfate in the urine was significantly higher than that of the control animals ; thus, 30〜45% of the given amount of thiothiamine was decomposed into sulfate within 48 hours. When 3-[2'-methyl-4'-aminopyrimidyl(5') ]-methyl-4-methyl-4-hydroxy-5β-hydroxyethylthiazolidine-2-thione (II), presumably an intermediate metabolite of thiothiamine, was given at the amount of 25 or 50 mg to rats, it was also decomposed to sulfate and excreted in the urine to the extent more than 70%, while the excretion of (II) was less than 20% of the given dosage, and excretion of (I) was 0.1 or 0.29 mg in the 24 hour urine. A small amount of (II) was detected in urine when thiothiamine was given. Degradation of thiothiamine to (I) and 2-mercapto-4-methyl-5β-hydroxyethylthiazole in rats was assumed to be such processes through presumed intermediate (II), as shown in the chart 2.

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[title in Japanese]

[in Japanese]

Article type: Article
1964 Volume 29 Issue 5 Pages 458-
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_458

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STUDIES ON THIOTHIAMINE : (XI) ISOLATION OF A PYRIMIDINE DERIVATIVE FROM THE URINE OF RATS AFTER INJECTION OF 2-METHYL-4-AMINO-5-AMINOMETHYLPYRIMIDINE

Chikataro KAWASAKI, Nobuko IRITANI

Article type: Article
1964 Volume 29 Issue 5 Pages 459-461
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_459

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The metabolite of 2-methyl-4-amino-5-aminomethylpyrimidine (I), when (I) was given parenterally to rats, was isolated from the urine : the urine was passed through a Zeolite column, and through an Amberlite IRC-50 column. The effluent from the Amberlite column was concentrated and extracted with ethanol. 2-Methyl-4-amino-5-pyrimidine carboxylic acid (II) from the ethanol extract was separated and identified by paper chromatography, absorption spectrography and electrophoresis. Its picrate also coincided with the picrate of (II) in mixed melting point and in elementary analysis.

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STUDIES ON THIOTHIAMINE : (XII) ISOLATION OF A PYRIMIDINE DERIVATIVE FROM 2-METHYL-4-AMINO-5-AMINOMETHYLPYRIMIDINE ON INCUBATION WITH RAT LIVER HOMOGENATE

Chikataro KAWASAKI, Nobuko IRITANI

Article type: Article
1964 Volume 29 Issue 5 Pages 462-465
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_462

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2-Methyl-4-amino-5-aminomethylpyrimidine (I), when incubated with a homogenate of rat liver for 3 hours, was degradated, but in an anaerobic condition, (I) was partly recovered unchanged. 2-Methyl-4-amino-5-pyrimidine carboxylic acid (II) from the reaction-mixture was separated by means of paper chromatography and identified as its picrate in comparison with an authentic sample. Another metabolite, presumed to be 2-methyl-4-amino-5-formylpyrimidine (III) was detected on paper chromatogram. Either uptake of oxygen or liberation of ammonia was not clearly demonstrated on incubation of the homogenate with (I) but N_2-saturation or addition of catron, inhibitor of amine-oxidase, markedly inhibited degradation of (I). Enzymatic degradation of (I) to (II) via (III) by rat liver homogenate is partly comparable to monoamine-oxidase but further degradation of (II) or entirely different metabolic pathway of (I) may occur, because (I) was quickly metabolized either by injection of (I) to rats or on incubation of (I) with the liver homogenate.

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BIOSYNTHESIS OF THIAMINE : (II) ISOLATION OF A PYRIMIDINE DERIVATIVE FROM THE LEAVES OF RICE PLANT

Chikataro KAWASAKI, Nobuko IRITANI

Article type: Article
1964 Volume 29 Issue 5 Pages 466-469
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_466

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The leaves of rice plant were extracted with 1-3% CCl_3COOH solution and the extract was passed through the columns of Amberlite IRA-410 and then of IRA-411. The amine substances from the effluent were adsorbed on the column of IRC-50.2-Methyl-4-amino-5-aminomethylpyrimidine (I) from the eluate was concentrated, purified by means of both paper chromatography and thin layer chromatography and then isolated as a picrate (mp. 227℃), which coincided with the picrate from the authentic sample. When rice-seeds in sand culture were germinated with addition of (I) and 4-methyl-5β-hydroxyethylthiazole (II)(each 10^<-4> or 10^<-6> moles to 1g of seeds), thiamine contents of seedling with (I) and (II) was higher than those without (I) and (II). (I) is most probably a precursor of thiamine during the process of its biosynthesis in rice-leaves.

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RADIOACTIVE SUBSTANCES ELIMINATED IN URINE, FOLLOWING INTRAVENOUS INJECTION OF RADIOACTIVE LIPOIC ACID : (II) SEPARATION OF PRINCIPAL METABOLITES OF LIPOIC ACID IN URINE BY PHENOL-EXTRACTION

Katsu TAKENOUCHI, Kazuo ASO, Yasuaki SHIRATORI, Shinichiro KAWASHIMA

Article type: Article
1964 Volume 29 Issue 5 Pages 470-479
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_470

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It was reported in previous paper that eleven ratioactive substances were detected in the urine of normal human subjects following intravenous injection of lipoic acid labelled with S^<35>, including a small amount of free lipoic acid. Further investigation was done to separate the main metabolic products in the urine by ethylacetate and phenol extraction and separated into fraction I to IV using Sephadex column. Fraction I and IV responded for Streptococcus faecalis R and thought to be lipoic acid derivatives.

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...

Article type: Article
1964 Volume 29 Issue 5 Pages 480-
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_480_1

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese]

Article type: Article
1964 Volume 29 Issue 5 Pages 480-481
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_480_2

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...

Article type: Article
1964 Volume 29 Issue 5 Pages 481-
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_481_1

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese]

Article type: Article
1964 Volume 29 Issue 5 Pages 481-
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_481_2

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...

Article type: Article
1964 Volume 29 Issue 5 Pages 481-482
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_481_3

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[title in Japanese]

[in Japanese]

Article type: Article
1964 Volume 29 Issue 5 Pages 482-
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_482_1

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1964 Volume 29 Issue 5 Pages 482-
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_482_2

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1964 Volume 29 Issue 5 Pages 482-
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_482_3

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1964 Volume 29 Issue 5 Pages 482-
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_482_4

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1964 Volume 29 Issue 5 Pages 482-483
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_482_5

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1964 Volume 29 Issue 5 Pages 483-
Published: May 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.5_483_1

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