Abstract
It is well known that the marked response of thymidine on the growth of the vitamin B_<12>-requiring strain, Lactobacillus leichmannii. In the present paper, additive effects of the other deoxyribonucleosides, 2'-deoxyadenosine and 2'-deoxyinosine were studied by L. leichmannii ATCC 7830,and the results are described. At first, it was found that positive influence of 2'-deoxypurinenucleosides on the growth of the strain was always demonstrated by paper chromatography and bioautography. The paper chromatography and bioautography for the separation and identification of 2'-deoxynucleosides and vitamin B_<12> were also successfully completed using 0.5〜2.5μg of 2'-deoxyadenosine and 0.05〜0.1mμg of vitamin B_<12> and water-saturated n-butanol as mobile phase.
