Abstract
Method of separation and detection of several antioxidants in vitamin A oil or in vegetable oil by thin layer chromatography was investigated. Butylhydroxyanisol (BHA), dibutylhydroxytoluene (BHT), isoamylgallate (IAC) and nordihydroguaiaretic acid (NDGA) in oils were extracted with an equal part of methanol. BHA and BHT in the extract were successfully separated with one-dimensional chromatography using silicagel as chromatographic plate and chloroform, methanol or petroleum ether as the developing solvent. In the case of separation of IAG and NDGA, the two-dimensional chromatography, using polyamide powder as chromatographic plate and methanol and chloroform as solvents, were applied. After separation, 20% phosphomolybdic acid solution in ethanol and ammonia vapour were used as colour developing reagents. Micro-quantities of antioxidants in vitamin A oil or in vegetable oil could be successfully separated and identified, by the above procedures.
