Abstract
JTC-3 cells were grown in YLE-media containing thiamine and its derivatives such as TPD, TATD, BTMP and CAT at high concentrations equivalent to 10 to 50 μg per ml of thiamine hydrochloride. The long-term tissue culture was performed in the case with thiamine and TPD. The effects of these compounds on the cell growth and the susceptibility of the cell to Poliovirus Type I were studied. As a result, it was recognized that TPD and TATD at high concentrations over 20 μg per ml had remarkable inhibitory effects on the cell growth, especially in the former, however, not so significant of both BTMP and CAT. On the other hand, thiamine showed no such effect on the cell growth, even after the longterm cultivation. The viral growth was slightly suppressed by the addition of both TPD and TATD. It would be realized that this suppression might be attributed to the reduced activity of the cells by these compounds.
