Abstract
For the separation of pyridoxal-5-phosphate from the other vitamin B_6 analogues, a preliminary examination was made on the thin layer chromatography. It was found that the properties of fluorescence and Gibbs' coloration with pyridoxal-5-phosphate and its analogues were dependent on the kind of adsorbent, and these methods might be applicable for the detection of pyridoxal-5-phosphate, only when the concentration of the sample above 10^<-4>M. Accordingly, a test solution of pyridoxal-5-phosphate for thin layer chromatography would require the concentration of 10^<-3>M at least. It was also clarified that dioxane・water (7 : 3) was the most suitable developing solvent so far examined for separation of pyridoxal-5-phosphate, pyridoxine, pyridoxal and pyridoxamine. The recovery rate of pyridoxal-5-phosphate applied to thin layer of cellulose was found to be 96.9%.
