Abstract
For the separation of pyridoxal-5-phosphate from the other vitamin B_6 analogues, a preliminary examination was made on the ion-exchange column chromatography using DEAE-, TEAE- SM-, and SE-cellulose. The satisfactory separation of pyridoxal-5-phosphate was accomplished with use of 0.01N HCl as elute for DEAE-cellulose, 0.01M acetate buffer (pH 4.4) for either TEAE- or SM-cellulose and 0.001M acetate buffer (pH 4.7) for SE-cellulose, respectivery. The recovery of pyridoxal-5-phosphate from DEAE-cellulose column was found to be about 97-99% and that from TEAE-cellulose column to be about 90〜98%.
