Abstract
Vitamin B_6 contents of Saccharomyces carlsbergensis were assayed microbiologically (Atkin : Ind. Eng. Chem. Anal. Ed. 15,141,1943) at various stages of the growth in the broth with or without thiamine. 1) In the broth without thiamine and pyridoxine, the vitamin B_6 content of cells reached to the maximal value (15〜20 mμg/mg) at the end of the lag phase and then dropped to 5〜7 mμg/mg at the logarithmic growth. 2) In the broth with thiamine only, the yeast began to grow after a prolonged lag phase, while the vitamin B_6 content continued to drop to the minimal value (1 mμg/mg) at the maximal growth, showing no peakpoint of the content. 3) In the broth with thiamine and pyridoxine, the yeast grew more rapidly than in other broths. The vitamin B_6 content of cells reached the highest peak (47 mμg/mg) after 6 hours but fell down to values as those low as in the broth 2) at the logarithmic growth. Vitamin B_6 was found in the broth after 6 hours but no vitamin B_6 was detected in it after 10 hours. 4) In the broth with pyridoxine, the yeast grew rapidly and its vitamin B_6 content fell down quickly.
