Abstract
A culture method for the isolation of anaerobic thiaminolytic bacilli from human feces was accomplished, according to Buchner's method of the exclusion of oxygen from culture plates, using pyrogallol and anhydrous sodium carbonate in tightly closed plates. This method was favorable for the development d superficial colonies as well as for daily observations, maintaining the necessary anaerobie state. This technique was found to be simple and easier than other methods in general use, for example, phosphorus jar method, so it seemed to be more suitable for dealing with many samples at the same time. The actual application of this method for the isolation was done by the two kinds of culture method. One was the direct inoculation of diluted materials onto agar plates and the other the indirect inoculation, namely the previous growth of bacilli in a liquid medium. The latter is more successful than the former.
