Abstract
It is well known that pantothenate stimulates the growth rate of yeast. Concerning with pantothenol, Snell discovered that the compound may response to the microorganism as a competitive inhibitor. Afterwards, Bird repeated the studies on the inhibitive properties of D-pantothenol in the microbioassy of pantothenate by Leuconostoc mesenteroides, and it was found that the microbial determination of pantothenol may be possible using the same strain. Recently, the present authors have attempted further investigation for the competitive ratio between both compounds, and it was realized that there is the competitive ratio of 200. Finally, it was demonstrated that the estimation of D-pantothenol can be successfully completed in the range of 0.5〜1.0 μg/2ml D-pantothenol.
