Abstract
The thiamine production from hydroxymethylpyrimidine (OMP) in the medium containing excess of thiazole by a mutant strain (thiazole-less) of Escherichia coli was proportional to a small amount of OMP (1-10 mμ moles) for a short time incubation. Therefore, the effects of the components of nucleic acid and their precursors on the thiamine production and conversion of these test materials to OMP were investigated using thiazole-less strain of E.coli. No other compounds than 2-methy-4-aminopyrimidine derivatives strictly similar to the structure of OMP gave significant effect on the thiamine synthesis while orotate and uridine had a little effect. Further, the conditions of OMP determination by baker's yeast suspensions containing glucose and thiazole was obtained and the conversion of purine derivatives to OMP base on the OMP determination method was tested by cell free extracts of E.coli. No any positive result was obtained.
