Abstract
The transport of vitamin B_6 in human erythrocyte was investigated, in vitro. The reaction mixture was consisted with the suspension of human erythrocyte in phosphate buffer containing glucose and various types of vitamin B_6,such as pyridoxine, pyridoxal, pyridoxamine and their phosphates. The amount transported in erythrocyte after incubation was determined by bioassay with Saccharomyces carlsbergensis. The rate and total amount of transportation in the erythrocyte increased according to the concentration of vitamin B_6 in the medium, linearly between 1-4μg/ml of pyridoxine and 1-160μg/ml of pyridoxal. The rate of transportation lowered in acidic pH range in the cases of pyridoxine and pyridoxamine, while in pyridoxal the effect of pH was scarcely observed. The concentration of erythrocyte in the suspension did not influenced on the transport. In the cases of vitamin B_6 phosphates, the transported amounts in erythrocyte were very small compared with the free types. These results lead to the conclusion that vitamin B_6 is transported into erythrocyte against a concentration gradient by energy-dependent mediated by a membrane carrier.
