Abstract
Studies were made on the pyridoxal phosphate content in erythrocyte after an incubation in phosphate buffer, containing glucose with free or phosphorylated forms of vitamin B_6. Pyridoxal phosphate did not increased whatever the concentrations of pyridoxine, pyridoxal or pyridoxamine in the reaction mixture. However, when pyridoxal phosphate was added, its content in erythrocyte increased moderately with the increase of given pyridoxal phosphate amount. With regard to the binding of pyridoxine or pyridoxal with ghost cells, the authors could not recognized the binding. The releasing of pyridoxal from pyridoxal phosphate by ghost cells to the extent of about 24%, and the reaction was inhibited by an addition of ATP.
