Abstract
Studies were made on the effect of phosphate on the transport of pyridoxine, pyridoxal and pyridoxamine in human erythrocyte. The rate of transfer of pyridoxine into erythrocyte markedly decreased in parallel with the amount of phosphate in the medium. As for pyridoxamine, it slightly decreased, while pyridoxal did not. Further, the decrease of phosphate concentration in the medium resulted in the diminution of pyridoxal phosphate in erythrocyte after an incubation with various forms of vitamin B_6. The transport of phosphate in human erythrocyte was investigated. Incubation of erythrocyte with glucose in phosphate medium resulted in accumulation of inorganic and organic phosphate along with lactic acid in erythrocyte. Lactic acid formed in erythrocyte was increased in parallel with the amount of phosphate in the medium. These results lead to the conclusion that the amount of vitamin B_6 transported in erythrocyte increases in accordance with the acceleration of glycolysis induced by phosphate.
