Abstract
Distribution of ^<35>S was studied in urine and organs of rabbit after an intravenous injection of thiamine-^<35>S. Urinary ^<35>S-compounds were fractionated into thiamine and its metabolites by paper chromatography. About 40% of administered ^<35>S was found in liver 30min. after injection. In testes, heart and brain, ^<35>S contents at 3 hrs. after injection were a little higher than those at 30min. after, while in lung and muscle, it decreased with the lapse of time. Thiamine and ^<35>S in plasma increased promptly after injection and it disappeared with the lapse of time. Thiamine level measured by thiochrome method was higher than that was calculated from ^<35>S until 1 hr. after injection, then it decreased more rapidly. In urine ^<35>S was fractionated in various fractions, such as thiamine, thiochrome, 4-methyl-5β-hydroxymethylthiazole, 4-methyl-5-carboxymethylthiazole and unknown substances. ^<35>S was detected also in bile. Percentages of urinary ^<35>S to administered dose during a period of 30 min., 3 and 5 hrs. after injection, were about 35,46 and 68,respectively. It was revealed that about 54% of ^<35>S, excreted in 5 hrs. urine was in thiamine molecule, while the remainder was in its metabolites, indicating that about one half of thiazole moiety degradated.
