Abstract
Metabolic pathway of BTDS to thiamine in human blood was investigated in vitro and in vivo, using acylthiochrome fluorometry. BTDS was found to be converted to thiamine mainly through the nonenzymatic reduction by erythrocytes and enzymatic deacylation by plasma. The deacylating activity of plasma was as high as 25 mg OBT per dl per hour at 37℃ and had the optimum pH at 7〜8. Similar enzymatic activities were also detected in homogenates of rat liver and intestine as well as Takadiastase. After oral administration of 300〜50O mg of BTDS on healthy subject, OBT or BTDS was not recognized in blood or urine, and it was concluded that absorbed BTDS was metabolized to thiamine. From these findings the metabolism of BTDS in blood was discussed.
