Abstract
Cells obtained from various sources were cultivated in growth media containing high concentrations of disulfide derivatives of thiamine, such as TPD, TTFD or BTDS. Effect of these derivatives on growth of the cells were investigated by counting of nuclei. The cells employed were as follows : HeLa cells, L cells, hamster kidney cells in primary and secondary monolayer cultures, and monkey (Macacus cynomolgus) kidney cells in primary monolayer cultures. Although growth-inhibitory effects were scarcely detected at concentration of 5μg/ml of TPD, cytopathic and growth-inhibitory effects were remarkable at concentrations higher than 30μg/ml of TPD. However TTFD did not show such notable effects as TPD did, furthermore, those of BTDS were the weakest at the same concentration. The results obtained above indicated that the similar effects of these derivatives were always found in any monolayer culture not only on normal cells, but also on malignant cells, on both sorts of cells whether they were epithelial or nonepithelial, moreover on mammalian cells generally. But growth of chick embryo heart tissues in plasma clot cultures was not checked even by high concentration (100μg/ml) of TPD.
