Abstract
Two methods of alumina-column chromatographic separation for the identification of vitamin D_2,5,6-trans-vitamin D_2,isocalciferol (isovitamin D_2), isotachysterol_2,tachysterol_2,lumisterol_2 and ergosterol were investigated. One involved the elution by n-hexane・ethyl ether (2 : 1) (method I), and the other was a gradient chromatography involving the elution by n-hexane・ethyl ether (4 : 1.3 : 1 and 2 : 1) (method II). Vitamin D_2 from tachysterol_2,5,6-trans-vitamin D_2 from isocalciferol and isotachysterol_2 from lumisterol_2 were not separated by the chromatography according to method I. However, the separations of vitamin D_2 from tachysterol_2 and isotachysterol_2 from lumisterol_2 were possible by the gas chromatography, so the identification of these compounds were possible by the application of the alumina-column chromatography and gas chromatography. The separative identification of 5,6-trans-vitamin D_2 from isocalciferol was possible by the chromatography according to method II.
